Proteomic analysis of right and left cardiac ventricles under aerobic conditions and after ischemia/reperfusion
Proteomic analysis of right and left cardiac ventricles under aerobic conditions and after ischemia/reperfusion
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DOI：
10.1002/pmic.201100604
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发表时间：
2012-08-01
期刊：
影响因子：
3.4
通讯作者：
Sawicki, Grzegorz
中科院分区：
文献类型：
--
作者：
Cadete, Virgilio J. J.;Lin, Han-bin;Sawicki, Grzegorz
缺血/再灌注(I/R)损伤是心血管干预的主要后果。研究I/R心脏左、右室蛋白质组的变化可能是揭示I/R所致心脏收缩功能障碍的病理机制的关键。分离的大鼠心脏在有氧条件下灌流，或进行25min全脑缺血再灌流30min。灌流结束后，用二维超声心动图分析左、右室心肌组织匀浆。心肌收缩功能和冠脉流量在I/R后显著降低。2DE和质谱仪分别鉴定出10个有氧左、右室差异显著的蛋白质点，8个有氧和I/R左室差异的蛋白质点，10个有氧和I/R右室差异的蛋白质点，10个I/R组差异不同的蛋白质点。这些蛋白点包括ATP合成酶β亚基、肌球蛋白轻链2、肌球蛋白重链片段、过氧化还蛋白-2和热休克蛋白，这些蛋白以前与心血管疾病有关。这些结果揭示了有氧条件下和I/R反应时左、右心室蛋白质组的差异，这有助于更好地理解I/R损伤。
Ischemia/reperfusion (I/R) injury is a major consequence of a cardiovascular intervention. The study of changes of the left and right ventricle proteomes from hearts subjected to I/R may be a key to revealing the pathological mechanisms underlying I/R-induced heart contractile dysfunction. Isolated rat hearts were perfused under aerobic conditions or subjected to 25 min global ischemia and 30 min reperfusion. At the end of perfusion, right and left ventricular homogenates were analyzed by 2DE. Contractile function and coronary flow were significantly reduced by I/R. 2DE followed by mass spectrometry identified ten protein spots whose levels were significantly different between aerobic left and right ventricles, eight protein spots whose levels were different between aerobic and I/R left ventricle, ten protein spots whose levels were different between aerobic and I/R right ventricle ten protein spots whose levels were different between the I/R groups. Among these protein spots were ATP synthase beta subunit, myosin light chain 2, myosin heavy chain fragments, peroxiredoxin-2, and heat shock proteins, previously associated with cardiovascular disease. These results reveal differences between proteomes of left and right ventricle both under aerobic conditions and in response to I/R that contribute to a better understanding of I/R injury.