Knock-down of OsDCL2 in rice negatively affects maintenance of the endogenous dsRNA virus, Oryza sativa endornavirus.
Knock-down of OsDCL2 in rice negatively affects maintenance of the endogenous dsRNA virus, Oryza sativa endornavirus.
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DOI：
10.1093/pcp/pcp167
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发表时间：
2010
影响因子：
4.9
通讯作者：
Syun-ichi Urayama;H. Moriyama;Nanako Aoki;Y. Nakazawa;Ryo Okada;Eri Kiyota;D. Miki;K. Shimamoto;T. Fukuhara
中科院分区：
文献类型：
--
作者：
Syun-ichi Urayama;H. Moriyama;Nanako Aoki;Y. Nakazawa;Ryo Okada;Eri Kiyota;D. Miki;K. Shimamoto;T. Fukuhara
一种内源性双链RNA (dsRNA)，最近被认为是dsRNA病毒Oryza sativa内RNA病毒(OsEV)，存在于许多栽培稻(Oryza sativa)品系中。检测到源自 OsEV dsRNA 的小 RNA，表明 RNA 沉默机制可识别 OsEV dsRNA。检查了五个 RNA 依赖性 RNA 聚合酶基因 (OsRDR1-OsRDR5) 或两个 Dicer 样蛋白基因 (OsDCL2 或 OsDCL3a) 的敲低 (KD) 系中 OsEV 的存在，以表征宿主 RNA 沉默系统与该 dsRNA 病毒传播之间的关系。在 OsRDR4-KD 或 OsDCL2-KD T(1) 系中未检测到 OsEV。我们尝试通过与携带 OsEV 的植物杂交将 OsEV 引入这些 KD 系中，因为 OsEV 通过花粉或卵子有效传递到 F(1) 植物。所有 OsRDR4-KD 但仅部分 OsDCL2-KD F(1) 植物含有 OsEV。一些 OsDCL2-KDF(1) 植物由携带 OsEV 和不含 OsEV 的细胞组成。这些结果表明 OsEV 的维持在 OsDCL2-KD 植物中不稳定。此外，OsDCL2-KD 植物中 OsEV 衍生的小干扰 RNA (vsiRNA) 的量相对于野生型有所增加。 vsiRNA 水平的增加可能会导致细胞分裂过程中 OsEV 不稳定。
An endogenous double-stranded RNA (dsRNA), which has recently been recognized as the dsRNA virus Oryza sativa endornavirus (OsEV), is found in many strains of cultivated rice (Oryza sativa). Small RNAs derived from OsEV dsRNA were detected, indicating that the RNA silencing machinery recognizes OsEV dsRNA. The existence of OsEV in knock-down (KD) lines of five genes of RNA-dependent RNA polymerase (OsRDR1-OsRDR5) or two genes of Dicer-like protein (OsDCL2 or OsDCL3a) was examined to characterize the relationship between the host RNA silencing system and the propagation of this dsRNA virus. OsEV was not detected in OsRDR4-KD or OsDCL2-KD T(1) lines. We attempted to introduce OsEV into these KD lines by crossing them with OsEV-carrying plants because of the efficient transmission of OsEV to F(1) plants via pollen or ova. All OsRDR4-KD but only some OsDCL2-KD F(1) plants contained OsEV. Some OsDCL2-KD F(1) plants consisted of OsEV-carrying and OsEV-free cells. These results suggest that the maintenance of OsEV is unstable in OsDCL2-KD plants. Furthermore, the amount of OsEV-derived small interfering RNA (vsiRNA) in the OsDCL2-KD plants increased relative to the wild type. This increased level of vsiRNA may cause OsEV instability during cell division.