Improved data processing for optical imaging of developing neuronal connectivity in the neonatal mouse barrel cortex
Improved data processing for optical imaging of developing neuronal connectivity in the neonatal mouse barrel cortex
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DOI：
10.1016/s1385-299x(01)00048-4
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发表时间：
2001-06-01
期刊：
影响因子：
--
通讯作者：
Nakamura, S
中科院分区：
文献类型：
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作者：
Itami, C;Samejima, K;Nakamura, S
使用电压敏感染料的光学记录方法已被证明对体内和体外神经元网络的分析都是有价值的。这种技术检测膜电位的变化，如掺入细胞质膜的电压敏感染料的吸收或荧光的变化。光学记录技术的可靠性取决于与染料相关的响应是否足够快以跟随电活性，以及响应是否或多或少与膜电位变化的幅度成比例。使用适当的成像系统和具有足够高的信噪比响应的染料可以实现高空间分辨率。因此，它是现在预计，这种方法将能够阐明更多的光的时空信息处理的新皮层电路。虽然FUJIX HR Deltaron 1700光学成像系统提供了相当高的时间（0.6 ms）和空间分辨率（在10倍放大率下为7 μ m），但该系统的一个缺点是。然而.是其相对较差的数据处理能力，因此，我们开发了一种协议，以提高信噪比，通过修改的计算算法的光学数据。因此，我们的特点是在丘脑皮层切片的光学反应，以找到发育的里程碑丘脑皮层和皮质内的连接在新生小鼠桶皮质。该方法已成功应用于丘脑皮层神经元连接的分析[8]。(C)2001 Elsevier Science B.V.保留所有权利。
Optical recording methods using voltage-sensitive dyes have proven valuable for the analysis of neuronal networks both in vivo and in vitro. This technique detects membrane potential changes as changes in the absorption or fluorescence of voltage-sensitive dyes incorporated into the cellular plasma membranes. The reliability of the optical recording technique is dependent on the dye-related response being fast enough to follow the electrical activity and of the response being more or less proportional to the amplitude of the membrane potential change. A high spatial resolution can be achieved using an appropriate imaging system and a dye with a response of sufficiently high signal-to-noise ratio. Thus, it is now anticipated that this method will be able to shed more light on the spatio-temporal information processing of neocortical circuitry. While the FUJIX HR Deltaron 1700 optical imaging system offers a reasonably high time (0.6 ms) and space-resolution (7 mum at 10X magnification), one drawback of this system. however. is its relatively poor data processing capabilities, We have therefore developed a protocol to improve the signal-to-noise ratio by modifying the calculation algorithm of the optical data. Consequently, we characterized optical responses in thalamocortical slices to find developmental landmarks of thalamocortical and intracortical connectivity in the neonatal mouse barrel cortex. Successful application of this method has been published on the analysis of thalamocortical glutamatergic connectivity [8]. (C) 2001 Elsevier Science B.V. All rights reserved.