EXPERIMENTAL DEMYELINATION PRODUCED BY THE A59-STRAIN OF MOUSE HEPATITIS-VIRUS
EXPERIMENTAL DEMYELINATION PRODUCED BY THE A59-STRAIN OF MOUSE HEPATITIS-VIRUS
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DOI：
10.1212/wnl.34.5.597
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发表时间：
1984-01-01
期刊：
影响因子：
9.9
通讯作者：
WEISS, SR
中科院分区：
文献类型：
--
作者：
LAVI, E;GILDEN, DH;WEISS, SR
用小鼠肝炎病毒（MHV）A59株（一种鼠冠状病毒）脑内接种4-6周龄C57 BL/6小鼠，产生双相疾病。急性肝炎和轻度脑膜脑炎，其次是亚急性痉挛性瘫痪与脱髓鞘病变的脑和脊髓所确定的Epon包埋的甲苯胺蓝染色切片和EM。用空斑法从急性期感染小鼠的血液、脑、脊髓和肝脏中培养出MHV-A59，而在慢性期则没有。免疫荧光法检测MHV-A59抗原至3 mo。感染后（PI）。从第7天至第5个月检测血清抗MHV-A59抗体。Pi. MHV-A59对脱髓鞘的诱导作用为进一步研究病毒诱导的脱髓鞘提供了一个合适的系统。
Intracerebral inoculation of 4-6-wk-old C57BL/6 mice with the A59 strain of mouse hepatitis virus (MHV), a murine coronavirus, produced biphasic disease. Acute hepatitis and mild meningoencephalitis were followed by subacute spastic paralysis with demyelinating lesions in the brain and spinal cord as determined by Epon-embedded toludine-blue-stained sections and by EM. MHV-A59 was cultured by plaque assay from the blood, brain, spinal cord and liver of infected mice during the acute phase, but not in the chronic stage. MHV-A59 antigen was detected by immunofluorescence (IF) until 3 mo. postinfection (PI). Serum anti-MHV-A59 antibodies were detected from 7 days to 5 mo. PI. The induction of demyelination by MHV-A59 provides a suitable system to study virus-induced demyelination further.