DETERMINATION OF PLASMA-VOLUME WITH INDOCYANINE GREEN IN MAN
DETERMINATION OF PLASMA-VOLUME WITH INDOCYANINE GREEN IN MAN
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DOI：
10.1016/0024-3205(93)90183-4
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发表时间：
1993-01-01
期刊：
影响因子：
6.1
通讯作者：
PETER, K
中科院分区：
文献类型：
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作者：
HALLER, M;AKBULUT, C;PETER, K
为探讨吲哚菁绿色（ICG）测定血浆容量（PV）的可行性，对23例健康人进行了重复性PV测定。一只手臂静脉注射ICG（0.25 mg/kg），另一只手臂抽取静脉血，用光密度计测量3 ~ 9分钟血浆样品的光密度。通过单指数外推法测定注射时间的ICG浓度。平均（SD）差异（MD）为-23 ml（183）或-0.6%（5.7%）。线性回归分析表明，PV_2 = 0.92.PV_1 +226（r=0.92）。PV值与其他研究的数据一致。在26例手术患者中，使用两种方法测定PV：1）与健康受试者相同，2）在全血（PVB）中使用该方法的改良。对于PVB测量，通过比色皿-密度计从动脉管线抽取血液。除了使用红细胞压积获得全血中ICG的血浆浓度外，计算与PV测定相同。在患者中，PV的MD为-53 ml（144）或-1.3%（4.3），PVB为-19 ml（161）或-0.3%（5.1）。比较PVB和PV显示MD = -113 ml（149）或-3.3%（4.2）。全血方法更容易执行，并将血液浪费减少到几乎为零。总之，ICG是测定PV的合适示踪剂。
We investigated the feasibility of using indocyanine green (ICG) for plasma volume (PV) determination in man. Duplicate PV measurements were carried out in 23 healthy subjects to test repeatability. ICG (0.25 mg/kg) was injected intravenously into one arm and venous blood was withdrawn from the opposite arm. Optical density of plasma samples from minute 3 to 9 was measured in a densitometer. ICG concentration at injection time was determined by monoexponential extrapolation. The mean (SD) difference (MD) was -23 ml (183) or -0.6% (5.7%). Linear regression revealed PV2=0.92.PV1+226 (r=0.92). The PV values corresponded well with data from other studies. In 26 surgical patients PV was determined using two methods: 1) the same as in healthy subjects and 2) using a modification of this method in whole blood (PVB). For PVB measurement blood was drawn through a cuvette-densitometer from an arterial line. Calculations were the same as in PV determination except for the use of hematocrit to achieve plasma concentrations of ICG from whole blood. In patients MD were -53 ml (144) or -1.3% (4.3) for PV and -19 ml (161) or -0.3% (5.1) for PVB. Comparing PVB and PV revealed MD = -113 ml (149) or -3.3% (4.2). The whole blood method is easier to perform and reduces blood waste to almost zero. In conclusion, ICG is a suitable tracer for PV determination.