Genomewide analysis of LATERAL ORGAN BOUNDARIES Domain gene family in Zea mays
Genomewide analysis of LATERAL ORGAN BOUNDARIES Domain gene family in Zea mays
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DOI：
10.1007/s12041-014-0342-7
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发表时间：
2014-04-01
影响因子：
1.5
通讯作者：
Zheng, Cheng-Chao
中科院分区：
文献类型：
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作者：
Zhang, Yue-Min;Zhang, Shi-Zhong;Zheng, Cheng-Chao
转录因子家族的研究是后基因组研究的重点。植物特有的不对称类LEAVES2(ASL)/侧向器官边界结构域(LBD)蛋白构成了一个主要的锌指结构域转录因子家族，调节植物的多种生物学过程。然而，对玉米(Zea Mays)的LBD基因知之甚少。在本研究中，共有44个LBD基因在玉米基因组中被鉴定出来，并与拟南芥中的LBD基因一起被聚为两大类(I和II)。预测的玉米LBD分布在全部10条不同密度的染色体上。此外，还对玉米LBD的基因结构进行了分析。利用基因芯片数据和qRT-PCR技术分析了玉米LBD基因在正常生长条件下的表达谱。这些结果表明，LBDS可能参与了玉米生理和发育过程的各个方面。据我们所知，这是首次对玉米LBD基因家族进行全基因组分析，这将为了解该基因家族的分类和可能的功能提供有价值的信息。
The investigation of transcription factor (TF) families is a major focus of postgenomic research. The plant-specific ASYMMETRIC LEAVES2-LIKE (ASL) / LATERAL ORGAN BOUNDARIES Domain (LBD) proteins constitute a major zinc-finger-like-domain transcription factor family, and regulate diverse biological processes in plants. However, little is known about LBD genes in maize (Zea mays). In this study, a total of 44 LBD genes were identified in maize genome and were phylogenetically clustered into two groups (I and II), together with LBDs from Arabidopsis. The predicted maize LBDs were distributed across all the 10 chromosomes with different densities. In addition, the gene structures of maize LBDs were analysed. The expression profiles of the maize LBD genes under normal growth conditions were analysed by microarray data and qRT-PCR. The results indicated that LBDs might be involved in various aspects of physiological and developmental processes in maize. To our knowledge, this is the first report of a genomewide analysis of the maize LBD gene family, which would provide valuable information for understanding the classification and putative functions of the gene family.