Dual Detection of Chromosomes and Microtubules by the Chromosomal Passenger Complex Drives Spindle Assembly
Dual Detection of Chromosomes and Microtubules by the Chromosomal Passenger Complex Drives Spindle Assembly
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DOI：
10.1016/j.devcel.2010.05.018
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发表时间：
2010-06-15
影响因子：
11.8
通讯作者：
Funabiki, Hironori
中科院分区：
文献类型：
--
作者：
Tseng, Boo Shan;Tan, Lei;Funabiki, Hironori
染色体依赖性纺锤体组装需要染色体募集和激活极光B，极光B是染色体乘客复合体（CPC）的激酶亚基。目前尚不清楚染色体活化的激酶如何在空间上传递信号以组织微米级纺锤体。我们发现CPC必须检测两种结构，染色体和微管，以支持爪蟾卵提取物的纺锤体组装。虽然Aurora B在中期的染色体上富集，但我们确定了一部分Aurora B靶向中期纺锤体并磷酸化微管结合底物。我们证明，染色体激活的极光B必须针对微管驱动纺锤体组装。此外，尽管cpc -微管相互作用可以激活Aurora B，从而进一步促进微管组装，但没有染色体就不会启动这种正反馈。我们认为CPC对染色体和微管的双重检测是围绕或仅围绕染色体组装纺锤体的关键步骤。
Chromosome-dependent spindle assembly requires the chromosomal recruitment and activation of Aurora B, the kinase subunit of the chromosomal passenger complex (CPC). It remains unclear how the chromosome-activated kinase spatially transmits signals to organize the micron-scale spindle. Here we reveal that the CPC must detect two structures, chromosomes and microtubules, to support spindle assembly in Xenopus egg extracts. While Aurora B is enriched on chromosomes in metaphase, we establish that a fraction of Aurora B is targeted to the metaphase spindle and phosphorylates microtubule-bound substrates. We demonstrate that chromosomally activated Aurora B must be targeted to microtubules to drive spindle assembly. Moreover, although the CPC-microtubule interaction can activate Aurora B, which further promotes microtubule assembly, this positive feedback is not initiated without chromosomes. We propose that the dual detection of chromosomes and microtubules by the CPC is a critical step in assembling spindles around and only around chromosomes.