Phylogenetic and Pathotypic Characterization of Newcastle Disease Viruses Circulating in South China and Transmission in Different Birds.
Phylogenetic and Pathotypic Characterization of Newcastle Disease Viruses Circulating in South China and Transmission in Different Birds.
复制标题
华南地区流行的新城疫病毒的系统发育和致病型特征及在不同鸟类中的传播
DOI：
10.3389/fmicb.2016.00119
复制
发表时间：
2016
影响因子：
5.2
通讯作者：
Ren T
中科院分区：
文献类型：
--
作者：
Kang Y;Xiang B;Yuan R;Zhao X;Feng M;Gao P;Li Y;Li Y;Ning Z;Ren T
纽卡斯尔病病毒（NDV）是一种高致病性病毒，自1948年以来在我国家禽中多次分离到，但其在禽类间的传播方式仍不清楚。鉴于各种野生鸟类都被认为是传播源，在这项研究中，我们对2013年12月至2014年2月期间从中国南方活禽市场（LBM）的鸡，鸭和鸽子中收集的23株NDV分离株进行了基因型和病理学特征分析，作为H7N9监测计划的一部分。为了模拟不同动物在LBM中的自然传播，我们选择了从不同鸟类中分离的三种代表性NDV，即GM，YF 18和GZ 289，以评估所指示的病毒在鸡，鸭和鸽子中的致病性和传播。此外，为了研究NDV在家禽中的复制和散毒，我们通过眼内和鼻内途径接种鸡、鸭和鸽子每种病毒106 EID 50。感染后8小时，将未接触过的接触组与接种过每种病毒的接触组圈养在一起，以监测接触传播。我们的研究结果表明，遗传多样性的病毒在LBM在中国南方的广东省和NDV从不同的鸟类有不同的组织嗜性和宿主范围时，在不同的鸟类传播。因此，我们建议对LBM进行持续的流行病学监测，以支持预防这些病毒在不同鸟类（特别是鸡）中的传播，并强调有必要研究病毒与宿主的关系。
Although Newcastle disease virus (NDV) with high pathogenicity has frequently been isolated in poultry in China since 1948, the mode of its transmission among avian species remains largely unknown. Given that various wild bird species have been implicated as sources of transmission, in this study we genotypically and pathotypically characterized 23 NDV isolates collected from chickens, ducks, and pigeons in live bird markets (LBMs) in South China as part of an H7N9 surveillance program during December 2013–February 2014. To simulate the natural transmission of different kinds of animals in LBMs, we selected three representative NDVs—namely, GM, YF18, and GZ289—isolated from different birds to evaluate the pathogenicity and transmission of the indicated viruses in chickens, ducks, and pigeons. Furthermore, to investigate the replication and shedding of NDV in poultry, we inoculated the chickens, ducks, and pigeons with 106 EID50 of each virus via intraocular and intranasal routes. Eight hour after infection, the naïve contact groups were housed with those inoculated with each of the viruses as a means to monitor contact transmission. Our results indicated that genetically diverse viruses circulate in LBMs in South China's Guangdong Province and that NDV from different birds have different tissue tropisms and host ranges when transmitted in different birds. We therefore propose the continuous epidemiological surveillance of LBMs to support the prevention of the spread of these viruses in different birds, especially chickens, and highlight the need for studies of the virus–host relationship.