INHIBITORY EFFECT OF SOME TRITERPENOID SAPONINS ON GLUCOSE-TRANSPORT IN TUMOR-CELLS AND ITS APPLICATION TO IN-VITRO CYTOTOXIC AND ANTIVIRAL ACTIVITIES
INHIBITORY EFFECT OF SOME TRITERPENOID SAPONINS ON GLUCOSE-TRANSPORT IN TUMOR-CELLS AND ITS APPLICATION TO IN-VITRO CYTOTOXIC AND ANTIVIRAL ACTIVITIES
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DOI：
10.1055/s-2006-959467
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发表时间：
1994-06-01
期刊：
影响因子：
2.7
通讯作者：
YAMASAKI, K
中科院分区：
文献类型：
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作者：
HASEGAWA, H;MATSUMIYA, S;YAMASAKI, K
采用2-脱氧-D-葡萄糖（2-DG）摄取法研究了几种三萜皂苷对埃利希瘤（EAT）细胞葡萄糖转运的影响。还研究了这些作用与对人T细胞系（MT-4）生长和MT-4细胞中人免疫缺陷病毒复制的作用的相关性。从人参根茎中分离得到的三七皂苷Ia。A. Meyer（五加科）以与2-DG（Ki = 0.32 mM）竞争的方式抑制2-DG摄取（IC 50 = 76.3 μ M），并以84.4 μ M的CC 50抑制MT-4细胞的生长，而它没有显示出任何显著的抗HIV-1活性。而从五加科植物姜参（Panax zingiberensis Wu et Feng）根茎中分离得到的姜苷R（1）则表现出一定的抗HIV-1活性，其抑制EAT细胞摄取2-DG（IC_（50）= 91.3 μ M）和MT-4细胞生长（CC_（50）= 46.2 μ M）的作用均上级姜黄素。
The effects of some triterpenoid saponins on glucose transport in Ehrlich ascites tumor (EAT) cells were examined by measuring 2-deoxy-D-glucose (2-DG) uptake. The correlation of the effects with those on the growth of a human T-cell line (MT-4) and the replication of human immunodeficiency virus in MT-4 cells was also studied. Chikusetsusaponin Ia isolated from rhizomes of Panax japonicus C. A. Meyer (Araliaceae) inhibited the 2-DG uptake (IC50 = 76.3 mu M) in a competitive fashion with respect to 2-DG (K-i = 0.32 mM) and the growth of MT-4 cells with CC50 of 84.4 mu M, whereas it did not show any significant anti-HIV-1 activity. In contrast, zingibroside R(1) isolated from rhizomes of Panax zingiberensis Wu et Feng (Araliaceae) showed some anti-HTV-1 activity, which was found to be superior to that of glycyrrhizin, as well as the inhibitory effects on the 2-DG uptake by EAT cells (IC50 = 91.3 mu M) and the growth of MT-4 cells (CC50 = 4 6.2 mu M).