Effects of Autologous Platelet-Rich Plasma on Cell Viability and Collagen Synthesis in Injured Human Anterior Cruciate Ligament
Effects of Autologous Platelet-Rich Plasma on Cell Viability and Collagen Synthesis in Injured Human Anterior Cruciate Ligament
复制标题
自体富血小板血浆对损伤人体前十字韧带细胞活力和胶原合成的影响
DOI：
10.2106/jbjs.i.01158
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发表时间：
2010-12-15
影响因子：
5.3
通讯作者：
Takahashi, Kazuhisa
中科院分区：
文献类型：
--
作者：
Fallouh, Louay;Nakagawa, Koichi;Takahashi, Kazuhisa
背景：富血小板血浆是血浆中血小板浓缩的一部分。据报道，它代表了促进组织修复的多种生长因子的来源。在前交叉韧带（ACL）缺陷患者的富血小板血浆的最终测试的预期，我们研究了自体富血小板血浆对人ACL细胞功能的影响在vitro.Methods：新鲜血液和ACL残余物从四个病人谁接受ACL重建手术。从血液样品制备贫血小板血浆和富血小板血浆。使用酶联免疫吸附测定法检测每种制剂中各种生长因子的浓度。在5%胎牛血清、5%贫血小板凝块释放物、5%富血小板凝块释放物或10%富血小板凝块释放物存在下培养分离的ACL细胞。将富血小板血浆和贫血小板血浆释放物应用于来自同一患者的自体ACL细胞。各组细胞活力和胶原蛋白合成进行了分析，和半定量基因表达测定I型和III型胶原蛋白也performed.Results：的主要生长因子（转化生长因子-β，血小板衍生生长因子，表皮生长因子，血管内皮生长因子）的浓度要高得多，在血小板丰富的凝块释放比血小板穷人凝块释放。在体外处理ACL细胞与血小板丰富的凝块释放导致细胞数量显着增加相比，血小板穷人凝块释放。仅由于增强的细胞增殖，富含血小板的凝块释放处理的细胞的总胶原蛋白产量显著高于缺乏血小板的凝块释放处理的细胞。有没有显着的效果，富含血小板的凝块释放治疗对基因表达的I型胶原蛋白，但III型胶原蛋白的表达显着增强的治疗与富含血小板的凝块releasate.Conclusions：这些结果表明，自体富含血小板的血浆可以提高ACL细胞的活力和功能在体外。
Background: Platelet-rich plasma is a fraction of plasma in which platelets are concentrated. It is reported to represent a source of multiple growth factors that promote tissue repair. In anticipation of the eventual testing of platelet-rich plasma in anterior cruciate ligament (ACL)-deficient patients, we examined the effect of autologous platelet-rich plasma on human ACL cell function in vitro.Methods: Fresh blood and ACL remnants were obtained from four patients who underwent ACL reconstruction surgery. Platelet-poor plasma and platelet-rich plasma were prepared from the blood samples. The concentrations of various growth factors in each preparation were tested with use of enzyme-linked immunosorbent assays. Isolated ACL cells were cultured in the presence of 5% fetal bovine serum, 5% platelet-poor clot releasate, 5% platelet-rich clot releasate, or 10% platelet-rich clot releasate. Platelet-rich plasma and platelet-poor plasma releasates were applied to the ACL cells from the same patient autologously. Cell viability and collagen synthesis in each group were analyzed, and semiquantitative gene-expression assays for type-I and III collagen were also performed.Results: The concentrations of the main growth factors (transforming growth factor-beta, platelet-derived growth factor, epidermal growth factor, and vascular endothelial growth factor) were much higher in platelet-rich clot releasate than in platelet-poor clot releasate. In vitro treatment of ACL cells with platelet-rich clot releasate resulted in a significant increase in cell number compared with platelet-poor clot releasate. Total collagen production by the platelet-rich clot releasate-treated cells was significantly higher than that of the platelet-poor clot releasate-treated cells only because of enhanced cell proliferation. There was no significant effect of platelet-rich clot releasate treatment on gene expression for type-I collagen, but expression of type-III collagen was significantly enhanced by the treatment with platelet-rich clot releasate.Conclusions: These results suggest that autologous platelet-rich plasma can enhance ACL cell viability and function in vitro.