Global analysis of chemical constituents in Shengmai injection using high performance liquid chromatography coupled with tandem mass spectrometry
Global analysis of chemical constituents in Shengmai injection using high performance liquid chromatography coupled with tandem mass spectrometry
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DOI：
10.1016/j.jpba.2015.08.022
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发表时间：
2016-01-05
影响因子：
3.4
通讯作者：
Yang, Hua
中科院分区：
文献类型：
--
作者：
Li, Fei;Cheng, Tao-fang;Yang, Hua
本研究旨在建立高效液相色谱-串联质谱联用技术对生脉注射液中化学成分进行综合分析的方法。在Kromasil 100-5C（18）色谱柱上对SMI进行定性分析，结果表明SMI中共有62个化合物被明确指定或初步鉴定，并通过HPLC-MS对14个皂苷、6个木脂素和1个l-冰片醇-7- o- [β - d -氨基呋喃基（1 - bbb6）]- β - d -葡萄糖吡喃苷等21个化合物进行了定量分析。此外，首次在SMI中鉴定并定量鉴定了来自麦冬的l-冰片醇-7- o- [β - d -apio-呋喃烷基（1 - bbb6）]- β - d -葡萄糖吡喃苷。方法验证结果表明，该方法简便、专属性强、可靠。所有化合物均具有良好的线性关系（r(2) >= 0.9992），浓度范围较宽，回收率为90.13 ~ 109.09%。该方法已成功应用于10批SMI样品的分析。所提出的方法可为SMI的质量控制提供有益而全面的参考，从而为SMI的质量控制和临床应用提供支持数据。(C) 2015 Elsevier B.V.版权所有
This study aimed to develop a specific and reliable method to comprehensively analyze the chemical constituents in Shengmai injection (SMI) using high performance liquid chromatography coupled with tandem mass spectrometry. The qualitative analysis of SMI was achieved on a Kromasil 100-5C(18) column, and the results demonstrated that a total of sixty-two compounds in SMI were unambiguously assigned or tentatively identified, and further, twenty-one compounds including fourteen saponins, six lignans and one L-borneol-7-O-[beta-D-apiofuranosyl (1 -> 6)]-beta-D-gluco-pyranoside were quantified by HPLC-MS. Furthermore, L-borneol-7-O-[beta-D-apio-furanosyl (1 -> 6)]-beta-D-glucopyranoside, originated from Radix ophiopogonis, was identified and quantified in SMI for the first time. The method validation results indicated that the methods were simple, specific and reliable. All the investigated compounds showed good linearity (r(2) >= 0.9992) with a relatively wide concentration range and acceptable recovery at 90.13-109.09%. Consequently, the developed methods were successfully applied to ten batches of SMI samples analysis. The proposed methods may provide a useful and comprehensive reference for the quality control of SMI, and thus to provide supporting data for the quality control and application of SMI clinically. (C) 2015 Elsevier B.V. All rights reserved.