Comparison of human whole blood, plasma, and serum matrices for the determination of perfluorooctanesulfonate (PFOS), perfluorooctanoate (PFOA), and other fluorochemicals
Comparison of human whole blood, plasma, and serum matrices for the determination of perfluorooctanesulfonate (PFOS), perfluorooctanoate (PFOA), and other fluorochemicals
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DOI：
10.1016/j.envres.2006.06.008
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发表时间：
2007-02-01
影响因子：
8.3
通讯作者：
Butenhoff, John L.
中科院分区：
文献类型：
--
作者：
Ehresman, David J.;Froehlich, John W.;Butenhoff, John L.
对人类接触全氟酸，包括全氟丁烷磺酸（PFBS）、全氟己磺酸（PFHS）、全氟辛烷磺酸（PFOS）和全氟辛酸（PFOA）的兴趣已导致在全血、血浆和血清中测量它们。然而，在这些不同的血液基质中测量值的比较还没有经过严格的研究，以允许跨基质比较。本研究评估了全血肝素（锂）和乙二胺四乙酸（EDTA）中PFBS、PFHS、PFOS和PFOA的浓度，血浆肝素和EDTA中PFBS和PFOA的浓度，以及血清（允许凝块的全血）。采集了3M公司18名志愿者的血样。所有分析样品制备均采用固相萃取法，分析采用高压液相色谱/串联质谱法完成。血清浓度范围为：PFBS的定量限（LOQ, 5 ng/mL）至25 ng/mL；PFHS的LOQ为5ng /mL ~ 75ng /mL；全氟辛烷磺酸的LOQ (5 ~ 880 ng/mL)；PFOA的LOQ（5或10 ng/mL）至7320 ng/mL。小于定量限的值不包括在矩阵的单个值的比率平均值的统计分析中。在大多数样本中，PFBS无法量化。全氟辛烷磺酸、全氟辛烷磺酸和全氟辛烷磺酸的血清与血浆比例为1:1，该比例与所测浓度水平无关。无论使用何种抗凝剂，血清或血浆与全血的比率都接近2:1。血浆、血清和全血之间的差异与红细胞的体积位移相对应，这表明氟化学物质不是在细胞内发现的，也不是附着在红细胞上的。(c) 2006爱思唯尔公司版权所有。
Interest in human exposure to perfluorinated acids, including perfluorobutanesulfonate (PFBS), perfluorohexanesulfonate (PFHS), perlluorooctanesulfonate (PFOS), and perfluorooctanoate (PFOA) has led to their measurement in whole blood, plasma and serum. Comparison of measurements in these different blood-based matrices, however, has not been rigorously investigated to allow for across-matrix comparisons. This research evaluated concentrations of PFBS, PFHS, PFOS, and PFOA in whole blood collected in heparin (lithium) and ethylenediamine tetraacetic acid (EDTA), plasma samples collected in heparin and EDTA, and serum (from whole blood allowed to clot). Blood samples were collected from 18 voluntary participants employed at 3M Company. Solid phase extraction methods were used for all analytical sample preparations, and analyses were completed using high-pressure liquid chromatography/ tandem mass spectrometry methods. Serum concentrations ranged from: limit of quantitation (LOQ, 5 ng/mL) to 25 ng/mL for PFBS; LOQ (5 ng/mL) to 75 ng/mL for PFHS; LOQ (5 ng/mL) to 880 ng/mL for PFOS; and LOQ (5 or 10 ng/mL) to 7320 ng/mL for PFOA. Values less than the LOQ were not included in the statistical analyses of the mean of the ratios of individual values for the matrices. PFBS was not quantifiable in most samples. Serum to plasma ratios for PFHS, PFOS, and PFOA were 1:1 and this ratio was independent of the level of concentrations measured. Serum or plasma to whole blood ratios, regardless of the anticoagulant used, approximated 2: 1. The difference between plasma and serum and whole blood corresponded to volume displacement by red blood cells, suggesting that the fluorochemicals are not found intracellularly or attached to the red blood cells. (c) 2006 Elsevier Inc. All rights reserved.