Incorporation of biodegradable electrospun fibers into calcium phosphate cement for bone regeneration.
Incorporation of biodegradable electrospun fibers into calcium phosphate cement for bone regeneration.
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DOI：
10.1016/j.actbio.2009.10.036
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发表时间：
2010-04
影响因子：
9.7
通讯作者：
Y. Zuo;Fang Yang;J. Wolke;Yubao Li;J. Jansen
中科院分区：
文献类型：
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作者：
Y. Zuo;Fang Yang;J. Wolke;Yubao Li;J. Jansen
固有的脆性和缓慢的降解是使用磷酸钙骨水泥（CPC）的主要缺点。为了解决这些问题，可生物降解的超细纤维被纳入CPC在这项研究中。以聚ε-聚己内酯（PCL）（PCL 12：1.1μm，PCL 15：1.4μm，PCL 18：1.9μm）和聚乳酸（PLLA 4：1.4μm）为原料，采用静电纺丝技术制备了4种纤维，并与CPC按纤维质量分数分别为1%、3%、5%和7%混合。将复合材料在模拟体液中孵育7 d后，通过重量法测定孔隙率、三点弯曲试验测定力学性能、微电脑形貌仪和扫描电镜观察其形貌。结果表明，超细纤维的掺入提高了CPC的抗断裂性能和孔隙率。复合材料的韧性随纤维含量的增加而增加，但不受纤维直径的影响。结果发现，掺入的纤维在CPC中形成了通道状的多孔结构。纤维降解后，复合骨水泥产生的空间和高孔隙率为骨组织生长提供了互连通道，并促进骨水泥吸收。因此，我们得出结论，这种静电纺丝纤维-CPC复合材料可能有利于用作骨填充物。
Inherent brittleness and slow degradation are the major drawbacks for the use of calcium phosphate cements (CPCs). To address these issues, biodegradable ultrafine fibers were incorporated into the CPC in this study. Four types of fibers made of poly(ε-polycaprolactone) (PCL) (PCL12: 1.1μm, PCL15: 1.4μm, PCL18: 1.9μm) and poly(l-lactic acid) (PLLA4: 1.4μm) were prepared by electrospinning using a special water pool technique, then mixed with the CPC at fiber weight fractions of 1%, 3%, 5% and 7%. After incubation of the composites in simulated body fluid for 7days, they were characterized by a gravimetric measurement for porosity evaluation, a three-point bending test for mechanical properties, microcomputer topography and scanning electron microscopy for morphological observation. The results indicated that the incorporation of ultrafine fibers increases the fracture resistance and porosity of CPCs. The toughness of the composites increased with the fiber fraction but was not affected by the fiber diameter. It was found that the incorporated fibers formed a channel-like porous structure in the CPCs. After degradation of the fibers, the created space and high porosity of the composite cement provides inter-connective channels for bone tissue in growth and facilitates cement resorption. Therefore, we concluded that this electrospun fiber-CPC composite may be beneficial to be used as bone fillers.