Participation of the dnaK and dnaJ gene products in phosphorylation of glutaminyl-tRNA synthetase and threonyl-tRNA synthetase of Escherichia coli K-12

Participation of the dnaK and dnaJ gene products in phosphorylation of glutaminyl-tRNA synthetase and threonyl-tRNA synthetase of Escherichia coli K-12
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dnaK和dnaJ基因产物参与大肠杆菌K-12谷氨酰胺酰-tRNA合成酶和苏氨酰-tRNA合成酶的磷酸化

DOI:
10.1128/jb.168.1.213-220.1986
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发表时间:
1986
影响因子:
3.2
通讯作者:
H. Itikawa
H. Itikawa
中科院分区:
生物学3区
文献类型:
--
作者:
M. Wada;K. Sekine;H. Itikawa

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大肠杆菌的热休克蛋白DNAK和DNAJ参与谷氨酰tRNA合成酶和苏氨酰tRNA合成酶的磷酸化。从42℃32PI标记的dna K7(Ts)和dna J2 59(Ts)突变细胞中提取的细胞蛋白进行双向凝胶电泳法分析时,与野生型细胞相比,没有观察到这些蛋白的磷酸化。
The heat shock proteins DnaK and DnaJ of Escherichia coli participate in phosphorylation of both glutaminyl-tRNA synthetase and threonyl-tRNA synthetase. When cellular proteins extracted from the dnaK7(Ts) and dnaJ259(Ts) mutant cells labeled with 32Pi at 42 degrees C were analyzed by two-dimensional gel electrophoresis, no phosphorylation of these proteins was observed when they were compared with those from wild-type cells.
DOI: --
发表时间: 1984
期刊: The Journal of biological chemistry
影响因子: --
作者:
Zylicz,M;Georgopoulos,C
通讯作者: Georgopoulos,C