OPERONS IN C-ELEGANS - POLYCISTRONIC MESSENGER-RNA PRECURSORS ARE PROCESSED BY TRANSSPLICING OF SL2 TO DOWNSTREAM CODING REGIONS
OPERONS IN C-ELEGANS - POLYCISTRONIC MESSENGER-RNA PRECURSORS ARE PROCESSED BY TRANSSPLICING OF SL2 TO DOWNSTREAM CODING REGIONS
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DOI：
10.1016/0092-8674(93)90139-h
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发表时间：
1993-05-07
期刊：
影响因子：
64.5
通讯作者：
BLUMENTHAL, T
中科院分区：
文献类型：
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作者：
SPIETH, J;BROOKE, G;BLUMENTHAL, T
已知六种秀丽隐杆线虫基因的信使核糖核酸（mRNAs）会反式剪接到SL2上。我们在此报道，在每个基因上游100 - 300个碱基对处都有一个方向相似的基因。我们提供的证据表明，这些基因簇中的基因是共转录的，并且下游的mRNAs是通过在多聚腺苷酸化位点切割以及反式剪接形成的。从一个三基因簇中，我们分离出了代表多顺反子RNAs以及在反式剪接产生的游离3'端多聚腺苷酸化的mRNAs的互补脱氧核糖核酸（cDNA）克隆，这表明多顺反子RNAs可以通过反式剪接进行加工。一些实验表明，SL2反式剪接是一个基因在操纵子中处于下游位置的结果。特别是，当一个接受SL1的基因被移到下游位置时，其mRNA大部分反式剪接到SL2上。文中还讨论了秀丽隐杆线虫基因共转录可能具有的调控意义。
The MRNAs of six C. elegans genes are known to be trans-spliced to SL2. We report here that a similarly oriented gene is located 100-300 bp upstream of each. We present evidence that the genes in these clusters are cotranscribed and downstream mRNAs are formed by cleavage at the polyadenylation site and trans-splicing. From one three-gene cluster we isolated cDNA clones representing both polycistronic RNAs and mRNAs polyadenylated at the free 3' end created by trans-splicing, suggesting that polycistronic RNAs can be processed by trans-splicing. Several experiments indicate that SL2 trans-splicing is a consequence of a gene's downstream location in an operon. In particular, when an SL1-accepting gene was moved to a downstream location, its mRNA was trans-spliced largely to SL2. The possible regulatory significance of cotranscription of C. elegans genes is discussed.