Regulation of distinct muscle behaviors controls the C. elegans male's copulatory spicules during mating
Regulation of distinct muscle behaviors controls the C. elegans male's copulatory spicules during mating
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DOI：
10.1016/s0092-8674(01)00600-6
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发表时间：
2001-12-14
期刊：
影响因子：
64.5
通讯作者：
Sternberg, PW
中科院分区：
文献类型：
--
作者：
Garcia, LR;Mehta, P;Sternberg, PW
我们通过细胞消融、分子遗传学和药理学方法证明，在秀丽隐杆线虫的雄性交配行为中，雄性通过调节周期性和长时间的针状肌收缩，将其交配针状体插入雌雄同体。不同的胆碱能神经元在针状肌中使用不同的乙酰胆碱受体和钙通道来调节这些收缩行为。PCB和PCC感觉神经元通过肌肉编码的UNC-68 ryanodine受体钙通道促进周期性收缩。SPC运动神经元通过EGL-19 l型电压门控钙通道触发延长的收缩。然后雄性性腺延长egl -19介导的延长肌肉收缩的持续时间。这种肌肉收缩的调节为解释动物如何启动、监测和维持行为运动程序提供了一个范例。
We demonstrate through cell ablation, molecular genetic, and pharmacological approaches that during C. elegans male mating behavior, the male inserts his copulatory spicules into the hermaphrodite by regulating periodic and prolonged spicule muscle contractions. Distinct cholinergic neurons use different ACh receptors and calcium channels in the spicule muscles to mediate these contractile behaviors. The PCB and PCC sensory neurons facilitate periodic contraction through muscle-encoded UNC-68 ryanodine receptor calcium channels. The SPC motor neurons trigger prolonged contraction through EGL-19 L-type voltage-gated calcium channels. The male gonad then lengthens the duration of EGL-19-mediated prolonged muscle contraction. This regulation of muscle contraction provides a paradigm to explain how animals initiate, monitor, and maintain a behavioral motor program.