UVC Light Prophylaxis for Cutaneous Wound Infections in Mice
UVC Light Prophylaxis for Cutaneous Wound Infections in Mice
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DOI：
10.1128/aac.00161-12
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发表时间：
2012-07-01
影响因子：
4.9
通讯作者：
Hamblin, Michael R.
中科院分区：
文献类型：
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作者：
Dai, Tianhong;Garcia, Barbara;Hamblin, Michael R.
人们早就知道紫外线C（UVC）具有很强的杀菌作用，但它作为一种感染治疗方法并没有得到太多发展。本研究探讨了UVC用于预防高度污染的浅表皮肤伤口发生感染的潜力。体外研究表明，病原菌铜绿假单胞菌和金黄色葡萄球菌在UVC照射下失活所需的剂量远低于对哺乳动物角质形成细胞产生类似效果所需的剂量。建立了感染生物发光铜绿假单胞菌和金黄色葡萄球菌的小鼠部分厚度皮肤擦伤模型。将大约10⁷个细菌细胞接种到小鼠背部面积为1.2×1.2厘米的伤口上。在细菌接种30分钟后给予UVC照射。研究发现，对于这两种细菌感染，单次辐射剂量为2.59焦耳/平方厘米的UVC照射使感染小鼠伤口中的细菌负荷比未处理小鼠伤口中的细菌负荷降低了约10倍（P < 0.00001）。此外，UVC照射使感染铜绿假单胞菌的小鼠存活率提高了58.3%（P = 0.0023），使感染金黄色葡萄球菌的小鼠伤口愈合率提高了31.2%（P < 0.00001）。在UVC照射处理的小鼠伤口中观察到了DNA损伤；然而，在UVC照射48小时后，这些损伤得到了广泛修复。这些结果表明，UVC可用于预防皮肤伤口感染。
UVC light has long been known to be highly germicidal but has not been much developed as a therapy for infections. This study investigated the potential of UVC light for the prophylaxis of infections developing in highly contaminated superficial cutaneous wounds. In vitro studies demonstrated that the pathogenic bacteria Pseudomonas aeruginosa and Staphylococcus aureus were inactivated at UVC light exposures much lower than those needed for a similar effect on mammalian keratinocytes. Mouse models of partial-thickness skin abrasions infected with bioluminescent P. aeruginosa and S. aureus were developed. Approximately 10(7) bacterial cells were inoculated onto wounds measuring 1.2 by 1.2 cm on the dorsal surfaces of mice. UVC light was delivered at 30 min after bacterial inoculation. It was found that for both bacterial infections, UVC light at a single radiant exposure of 2.59 J/cm(2) reduced the bacterial burden in the infected mouse wounds by approximately 10-fold in comparison to those in untreated mouse wounds (P < 0.00001). Furthermore, UVC light increased the survival rate of mice infected with P. aeruginosa by 58.3% (P = 0.0023) and increased the wound healing rate in mice infected with S. aureus by 31.2% (P < 0.00001). DNA lesions were observed in the UVC light-treated mouse wounds; however, the lesions were extensively repaired by 48 h after UVC light exposure. These results suggested that UVC light may be used for the prophylaxis of cutaneous wound infections.