Real-time spectroscopic analysis of ligand-receptor dynamics.
Real-time spectroscopic analysis of ligand-receptor dynamics.
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DOI：
10.1146/annurev.bb.16.060187.002403
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
L. Sklar
中科院分区：
文献类型：
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作者：
L. Sklar
前景与概述.....·.....···......···...... 479流式细胞术481游离和结合配体的鉴别481校准和平衡结合分析483动力学方法484 D！sc：内化配体的免疫486限制488荧光光谱分析488游离和结合配体的区分489结合和内化配体的区分496配体-受体动力学和细胞活化497荧光配体497占据-响应关系50 I模拟配体-受体动力学及其在细胞活化中的作用502
PERSPECTIVES AND OVERVIEW ....•........••• •.....•••......•• •...... 479 FLOW CYTOMETRY 481 Discrimination of Free and Bound Ligand 481 Calibration and Equilibrium Binding Analysis 483 Kinetic Methods 484 D!sc:im�nation of Internalized Ligand 486 LimitatIOns 488 SPECTROFLUORIMETRIC ANALySIS 488 Discrimination of Free and Bound Ligand 489 Discrimination of Bound and Internalized Ligand 496 LIGAND-RECEPTOR DYNAMICS AND CELL ACTIVATION 497 Fluorescent Ligands 497 Occupancy-Response Relationships 50 I Modeling Ligand-Receptor Dynamics and Its Role in Cell Activation 502