NADPH oxidase subunit gp91phox:: a proton pathway
NADPH oxidase subunit gp91phox:: a proton pathway
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DOI：
10.1007/bf01289411
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发表时间：
2001-01-01
期刊：
影响因子：
2.9
通讯作者：
Henderson, LM
中科院分区：
文献类型：
--
作者：
Henderson, LM
NADPH氧化酶产生超氧化物是一个产电过程，导致细胞膜电位快速去极化。H+离子通过花生四烯酸可激活的、Zn 2+可激活的HC途径的流出伴随着电子的流出并提供必要的电荷补偿。H+通量的抑制导致超氧化物生成的抑制。蛋白gp 91（phox）是NADPH氧化酶的跨膜组分，已证明能够在稳定的CHO细胞系CHO 91中充当NADPH氧化酶相关H+通道。N-末端230个氨基酸含有蛋白质形成Ht通道所需的所有氨基酸，特别是组氨酸115对gp 91（phox）传导H+离子的能力很重要。CHO 91细胞的外向电流的记录，在全细胞配置，表明gp 91（phox）也能够作为一个电压门控的He电导通路。电压引起的外向电流，从野生型和突变，和花生四烯酸激活的高通量之间的属性的相似性强烈表明，这些H+途径是相同的。在最近鉴定的gp 91（phox）的同源物中，迄今为止只有NOH-1 S被证明也是Ht传导途径。
The generation of superoxide by the NADPH oxidase is an electrogenic process resulting in a rapid depolarisation of the membrane potential of the cell. The efflux of H+ ions through an arachidonate-activatable, Zn2+-inhibitable HC pathway accompanies the efflux of electrons and provides the necessary charge compensation. Inhibition of H+ flux leads to inhibition of superoxide generation. The protein gp91(phox), a transmembrane component of the NADPH oxidase, was demonstrated to be capable of acting as the NADPH oxidase-associated H+ channel in a stable CHO cell line, CHO91. The N-terminal 230 amino acids contain all that is required for the protein to form an Ht channel and specifically histidine 115 is important to the ability of gp91(phox) to conduct H+ ions. The recording of outward currents from CHO91 cells, in the whole-cell configuration, demonstrated that gp91(phox) is also capable of functioning as a voltage-gated He conductance pathway. The similarity in properties between voltage-elicited outward currents, from both wild type and the mutations, and the arachidonate-activated Hi flux strongly suggests that these H+ pathways are one in the same. Among the recently identified homologues of gp91(phox) only NOH-1S has so far been demonstrated to also act as an Ht conductance pathway.