Characterization of a novel gene related to antibiotic susceptibility in Pseudomonas aeruginosa
Characterization of a novel gene related to antibiotic susceptibility in Pseudomonas aeruginosa
复制标题
铜绿假单胞菌中与抗生素敏感性相关的新基因的表征
DOI：
10.1038/ja.2011.111
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发表时间：
2011-12
影响因子：
3.3
通讯作者：
Liang, Haihua
中科院分区：
文献类型：
--
作者：
Shen, Lixin;Ma, Yanling;Liang, Haihua
铜绿假单胞菌是一种对临床治疗中使用的大量抗生素具有增加的内在耐药性的人类病原体。因此，了解铜绿假单胞菌的耐药机制和开发治疗方案具有深远的意义。我们实验室之前的工作表明，在亚抑制浓度（SICs）的四环素（泰特）存在下，分离出了几种phzA 1 B1 C1 D1 E1 F1 G1（phzA 1）操纵子表达改变的突变体。本研究探讨PA 0011基因在介导泰特SIC时phzA 1表达中的作用。PA 0011基因通过控制细胞被膜和外膜的合成来编码2-OH-月桂酰转移酶。我们发现PA 0011突变株对几种不同的抗生素和环境胁迫敏感。在PA 0011突变体的互补恢复这些表型野生型水平。此外，PA 0011基因的表达，如通过荧光素酶报告基因监测的，在抗生素的SIC中增加。事实上，与野生型PAO 1相比，PA 0011基因的表达在pqsR和pqsH突变体中增加了约三倍。然而，PA 0011基因负调控群体感应（QS）系统。综上所述，这些数据表明PA 0011参与铜绿假单胞菌对抗菌剂的敏感性，并且其敏感性效应可能部分依赖于QS表达的增加。
Pseudomonas aeruginosa is a human pathogen with increased intrinsic resistance to a large number of antibiotics used in clinical therapy. Therefore, understanding the mechanisms of resistance and developing therapy alternatives for P. aeruginosa are of profound importance. Previous work from our laboratory demonstrated that several mutants have isolated with altered expression of the phzA1B1C1D1E1F1G1 (phzA1) operon in the presence of sub-inhibitory concentrations (SICs) of tetracycline (TET). The present study investigates the roles of the PA0011 gene in mediating phzA1 expression at SIC of TET. The PA0011 gene encodes 2-OH-lauroytransferase by controlling the synthesis of the cell envelope and the outer membrane. We found that the PA0011 mutant strain was susceptible to several different antibiotics and environmental stresses. Complementation in the PA0011 mutant restored these phenotypes to wild-type levels. In addition, expression of the PA0011 gene, as monitored through a luciferase reporter, is increased at SICs of antibiotics. Indeed, the expression of the PA0011 gene increased about threefold in pqsR and pqsH mutants compared with the wild-type PAO1. However, the PA0011 gene negatively regulates the quorum sensing (QS) system. Taken together, these data suggest that PA0011 is involved in susceptibility to antimicrobial agents in P. aeruginosa, and that its susceptibility effect maybe partly dependent on increased QS expression.
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影响因子：
4.9
作者：
Doetsch, Andreas;Becker, Tanja;Haeussler, Susanne
通讯作者：
Haeussler, Susanne
影响因子：
3.6
作者：
Diggle, SP;Winzer, K;Williams, P
通讯作者：
Williams, P
DOI：
10.1038/nbt1183-784
发表时间：
1983-01-01
期刊：
BIO-TECHNOLOGY
影响因子：
--
作者：
SIMON, R;PRIEFER, U;PUHLER, A
通讯作者：
PUHLER, A
影响因子：
4.9
作者：
ANGUS, BL;CAREY, AM;HANCOCK, REW
通讯作者：
HANCOCK, REW
DOI：
10.1073/pnas.77.12.7347
发表时间：
1980-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
DITTA, G;STANFIELD, S;HELINSKI, DR
通讯作者：
HELINSKI, DR