Near-infrared nuclear markers for Drosophila imaging.
Near-infrared nuclear markers for Drosophila imaging.
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DOI：
10.17912/micropub.biology.000639
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发表时间：
2022
影响因子：
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通讯作者：
Irvine, Kenneth D
中科院分区：
文献类型：
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作者：
Pan, Yuanwang;Rauskolb, Cordelia;Irvine, Kenneth D
用于活体成像的核标记物可用于计数和跟踪细胞，可视化细胞分裂，以及检查通过进入或离开细胞核控制的蛋白质的调节。近红外荧光蛋白具有优于较短波长荧光蛋白的优势，包括降低的光毒性、较少的光散射和能够进行实时成像。我们已经构建并测试了表达组蛋白H2Av iRFP融合蛋白的转基因果蝇，并证实它们可用于标记处于多个发育阶段的固定和活组织中的细胞核。
Nuclear markers for live imaging are useful for counting and tracking cells, visualizing cell division, and examining the regulation of proteins that are controlled via entry or exit from the nucleus. Near-infrared fluorescent proteins have advantages over shorter wavelength fluorescent proteins, including reduced phototoxicity, less light scattering, and enabling multicolor live imaging. We have constructed and tested transgenic Drosophila expressing Histone H2Av iRFP fusion proteins, and confirmed that they can be used to label nuclei in both fixed and live tissue at multiple stages of development.