A novel acute anemia model for pharmacological research in mice by compelled acute exercise
A novel acute anemia model for pharmacological research in mice by compelled acute exercise
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通过强制急性运动建立小鼠药理学研究的新型急性贫血模型
DOI：
10.1038/aps.2009.161
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发表时间：
2009-12-01
影响因子：
8.2
通讯作者：
Du, Guan-hua
中科院分区：
文献类型：
--
作者：
Liu, Qing-shan;Wang, Jin-hua;Du, Guan-hua
目的：通过强迫运动建立一种新的急性贫血模型。方法：采用电刺激法强迫成年雄性小鼠进行30 min的急性运动，运动后立即从尾部采血检测。结果：力竭小鼠的红细胞计数、血红蛋白浓度和红细胞压积均低于安静小鼠。该模型符合诊断标准。作为急性贫血模型的机制，发现模型小鼠中RBC的渗透脆性与正常小鼠相比增加，并且模型小鼠中RBC的变形指数、取向指数、小变形指数、变形性和取向性降低。血浆谷胱甘肽（GSH）水平降低。结论：急性强迫性运动对大鼠贫血模型的影响，表明该模型的建立是成功的。红细胞的氧化损伤和血液流变学的改变是造成该模型急性贫血的部分原因。
Aim:To generate a novel acute anemia model by compelled exercise.Methods:Young adult male mice were compelled to perform an acute exercise by electric stimulation for 30 min. Blood was taken from their tails to be used for detection immediately after exercise.Results:It was found that the red blood cell (RBC) count, hemoglobin concentration and hematocrit of exhausted mice were lower than that of sedentary mice without electric stimulation. The model is in line with the diagnostic criteria. As the mechanisms of the acute anemia model, it was found that the osmotic fragility of the RBC in model mice increased compared to that of normal mice, and that the deformation index, orientation index, small deformation index, deformability and orientation of RBCs in the model mice were reduced. The glutathione (GSH) levels in blood plasma were decreased. The liver, spleen, kidney had no detectable changes.Conclusion:To the best of our knowledge, these effects represent the success of a novel anemia model by compelled acute exercise. The oxidative damages on RBC and hemorheological changes are in part the causes of acute anemia in this model.