Increased expression of transcription initiation factor IIB after rat traumatic brain injury
Increased expression of transcription initiation factor IIB after rat traumatic brain injury
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DOI：
10.1007/s10735-011-9330-x
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发表时间：
2011-05
影响因子：
3.2
通讯作者：
Zhiqiang Liu;Dongling Wang;Bai Shao;Xiaohong Wu;Jian Xu;Q. Lu;Yidan Wang;Chunmiao Li;A. She
中科院分区：
文献类型：
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作者：
Zhiqiang Liu;Dongling Wang;Bai Shao;Xiaohong Wu;Jian Xu;Q. Lu;Yidan Wang;Chunmiao Li;A. She
TFIIB蛋白是一种通用的转录起始因子，在前起始复合体（PIC）中起关键作用，并选择转录起始位点。然而，其在中枢神经系统（CNS）中的分布和功能尚不清楚。本研究主要探讨TFIIB在创伤性脑损伤（TBI）中的表达及细胞定位。Western blot分析显示，TFIIB存在于正常大鼠大脑皮层。逐渐升高，第5天达高峰，随后下降。重要的是，更多的TFIIB与大量增殖的星形胶质细胞和小胶质细胞共定位。Western blot检测结果显示，伤后5天也是增殖高峰，PCNA表达升高。重要的是，损伤诱导的TFIIB表达由增殖细胞核抗原（增殖细胞标记物）共同标记。这些数据表明，TFIIB可能涉及星形胶质细胞和小胶质细胞的增殖和神经功能的恢复。但其内在机制尚不清楚。需要进一步的研究来证实脑损伤后TFIIB的确切作用。
The protein TFIIB is a general transcription initiation factor that plays a pivotal role in the preinitiation complex (PIC) and selects the transcription initiation site. However, its distribution and function in the central nervous system (CNS) remains unclear. In the present study, we mainly investigated the expression and cellular localization of TFIIB during traumatic brain injury (TBI). Western blot analysis revealed that TFIIB was present in normal rat brain cortex. It gradually increased, reached a peak at the 5th day after TBI, and then decreased. Importantly, more TFIIB was colocalized with astrocytes and microglia, which are largely proliferated. In addition, Western blot detection showed that the 5th day post injury was also the proliferation peak indicated by the elevated expression of PCNA. Importantly, injury-induced expression of TFIIB was colabelled by proliferating cell nuclear antigen (proliferating cells marker). These data suggested that TFIIB may be implicated in the proliferation of astrocytes and microglia and the recovery of neurological outcomes. But the inherent mechanisms remained unknown. Further studies are needed to confirm the exact role of TFIIB after brain injury.