High-resolution episcopic microscopy (HREM): A useful technique for research in wound care
High-resolution episcopic microscopy (HREM): A useful technique for research in wound care
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DOI：
10.1016/j.aanat.2014.10.012
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发表时间：
2015-01-01
影响因子：
2.2
通讯作者：
Weninger, Wolfgang J.
中科院分区：
文献类型：
--
作者：
Geyer, Stefan H.;Tinhofer, Ines E.;Weninger, Wolfgang J.
分析皮肤替代材料的三维（3D）纹理并评估其覆盖皮肤缺陷后的性能，对于改进其设计、优化外科手术程序和植入后伤口治疗方案至关重要。在这里，我们探索了最近开发的高分辨率主教显微镜（HREM）方法的能力，用于生成数字体积数据，允许对天然和植入的胶原-弹性蛋白矩阵进行结构3D分析。我们使用HREM可视化天然胶原基质和角质形成细胞植入的胶原基质。在第二步中，我们可视化了用于覆盖猪模型皮肤缺陷的分裂皮肤移植物植入后基质的外观和血管重建。为此，HREM数据来自于术后5、10和15天的活检。在所有情况下，HREM数据的高质量和高分辨率与相对较大的视场相结合，证明了通过使用快速体绘制算法来可视化精确的光纤结构是足够的。在植入角质形成细胞的基质中精确分析角质形成细胞的三维分布以及植入基质中发芽血管的详细拓扑结构是可行的。我们的研究结果表明，高分辨率显微镜可以适应作为评估胶原-弹性蛋白材料的工具，在体外和体内。(C) 2014 Elsevier GmbH版权所有。
Analysing the three-dimensional (3D) texture of skin substitute materials and evaluating their performance after covering skin defects is essential for improving their design and for optimising surgical procedures and post implantation wound treatment regimes. Here we explore the capacities of the recently developed High-resolution episcopic microscopy (HREM) method for generating digital volume data that permit structural 3D analysis of native and implanted collagen-elastin matrices.We employed HREM to visualise native collagen matrices and collagen matrices seeded with keratinocytes. In a second step, we visualised the appearance and the revascularisation of the matrices after their implantation beneath split skin grafts Used for covering skin defects in the porcine model. For this, HREM data were generated from biopsies harvested 5, 10, and 15 days after surgery.In all instances, the high quality and resolution of the HREM data in combination with the relative large field of view proved to be sufficient for visualizing the exact fibre architecture by employing quick volume rendering algorithms. Precise analysis of the 3D distribution of keratinocytes in the matrices populated with keratinocytes and of the detailed topology of the sprouting blood vessels in the implanted matrices was feasible.Our results show that high-resolution episcopic microscopy can be adapted to serve as a tool for evaluating collagen-elastin materials ex- and in vivo. (C) 2014 Elsevier GmbH. All rights reserved.