Mode of action of plantaricin MG, a bacteriocin active against Salmonella typhimurium
Mode of action of plantaricin MG, a bacteriocin active against Salmonella typhimurium
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DOI：
10.1002/jobm.201000130
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发表时间：
2010-12-01
影响因子：
3.1
通讯作者：
Wang, Hui
中科院分区：
文献类型：
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作者：
Gong, Han-Sheng;Meng, Xiang-Chen;Wang, Hui
植物乳杆菌素MG（Plantaricin MG）是从中国传统发酵奶油中分离得到的一种分子量为2，180 Da的细菌素，由植物乳杆菌KLDS 1.0391产生。植物菌素MG不仅对革兰氏阳性菌显示出广泛的抑制活性，而且对革兰氏阴性菌包括单核细胞增生李斯特菌和鼠伤寒沙门氏菌也显示出广泛的抑制活性。植物菌素MG对S.本文报道了鼠伤寒沙门氏菌。将植物菌素MG加入到S.鼠伤寒沙门氏菌的跨膜电位（Δ psi）和pH梯度（Δ pH）均消失。通电膜，获得后，加入葡萄糖，更容易受到植物霉素MG的行动，导致释放细胞内K+离子，无机磷酸盐，ATP和紫外线吸收材料。这些数据表明，质子动力的存在促进植物菌素MG与通电细胞的细胞质膜的相互作用，导致孔形成，其允许离子流出，从而确保有效杀死靶细菌。
Plantaricin MG is a 2,180-Da bacteriocin produced by Lactobacillus plantarum KLDS1.0391, which was isolated from Chinese traditional fermented cream. Plantaricin MG showed a broad inhibitory activity against not only Gram-positive bacteria but also Gram-negative bacteria including Listeria monocytogenes and Salmonella typhimurium. The mode of action of plantaricin MG on S. typhimurium was reported in this article. The addition of plantaricin MG to energized cells of S. typhimurium dissipated both, the transmembrane potential (Delta psi) and the pH gradient (Delta pH). Energized membrane, obtained after the addition of glucose, was more susceptible to plantaricin MG action, leading to the release of intracellular K+ ions, inorganic phosphate, ATP and UV-absorbing materials. These data suggest that the presence of a proton motive force promotes the interaction of plantaricin MG with the cytoplasmic membrane of energized cells, leading to pores formation which allows the efflux of ions, thereby ensuring efficient killing of target bacteria.