Soluble CD14 Enhances the Response of Periodontal Ligament Stem Cells to P. gingivalis Lipopolysaccharide.
Soluble CD14 Enhances the Response of Periodontal Ligament Stem Cells to P. gingivalis Lipopolysaccharide.
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DOI：
10.1371/journal.pone.0160848
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Rausch-Fan X
中科院分区：
文献类型：
--
作者：
Andrukhov O;Andrukhova O;Özdemir B;Haririan H;Müller-Kern M;Moritz A;Rausch-Fan X
牙周膜干细胞 (PDLSC) 缺乏膜 CD14，CD14 是通过 Toll 样受体 (TLR) 4 进行脂多糖 (LPS) 信号传导的重要组成部分。在本研究中，我们研究了可溶性 CD14 对人类 PDLSC 对牙龈卟啉单胞菌 (P.) LPS 反应的影响。在存在或不存在可溶性 CD14 (sCD14) 的情况下用牙龈卟啉单胞菌 LPS 刺激人 PDLSC (hPDLSC)，并测量白细胞介素 (IL)-6、趋化因子 C-X-C 基序配体 8 (CXCL8) 和趋化因子 C-C 基序配体 2 (CCL2) 的产生。将牙龈卟啉单胞菌 LPS 的反应与 TLR4 激动剂大肠杆菌 LPS 和 TLR2 激动剂 Pam3CSK4 的反应进行比较。 sCD14 显着增强了 hPDLSC 对牙龈卟啉单胞菌 LPS 和大肠杆菌 LPS 的反应。在没有 sCD14 的情况下，hPDLSC 对两种 LPS 的反应没有观察到显着差异。与 Pam3CSK4 相比，这些反应明显较低。在sCD14存在的情况下，hPdLSC对牙龈卟啉单胞菌LPS的反应明显高于对大肠杆菌LPS的反应，并且与Pam3CSK4的反应相当。 sCD14 强烈增强了 hPdLSC 对细菌 LPS 的反应。 sCD14 的局部水平可能是调节宿主对牙周病原体反应的重要因素。
Periodontal ligament stem cells (PDLSCs) are lacking membrane CD14, which is an important component of lipopolysaccharide (LPS) signaling through toll-like receptor (TLR) 4. In the present study we investigated the effect of soluble CD14 on the response of human PDLSCs to LPS of Porphyromonas (P.) gingivalis. Human PDLSCs (hPDLSCs) were stimulated with P. gingivalis LPS in the presence or in the absence of soluble CD14 (sCD14) and the production of interleukin (IL)-6, chemokine C-X-C motif ligand 8 (CXCL8), and chemokine C-C motif ligand 2 (CCL2) was measured. The response to P. gingivalis LPS was compared with that to TLR4 agonist Escherichia coli LPS and TLR2-agonist Pam3CSK4. The response of hPDLSCs to both P. gingivalis LPS and E. coli LPS was significantly enhanced by sCD14. In the absence of sCD14, no significant difference in the hPDLSCs response to two kinds of LPS was observed. These responses were significantly lower compared to that to Pam3CSK4. In the presence of sCD14, the response of hPdLSCs to P. gingivalis LPS was markedly higher than that to E. coli LPS and comparable with that to Pam3CSK4. The response of hPdLSCs to bacterial LPS is strongly augmented by sCD14. Local levels of sCD14 could be an important factor for modulation of the host response against periodontal pathogens.