ROLE OF GENE DELETION IN THE IMMUNOGLOBULIN HEAVY-CHAIN SWITCH
ROLE OF GENE DELETION IN THE IMMUNOGLOBULIN HEAVY-CHAIN SWITCH
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DOI：
10.1038/283351a0
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发表时间：
1980-01-01
期刊：
影响因子：
64.8
通讯作者：
BENTLEY, DL
中科院分区：
文献类型：
--
作者：
RABBITTS, TH;FORSTER, A;BENTLEY, DL
小鼠H链亚类γ 2b、γ 2a和γ 1的基因排列通过Southern杂交方法进行检测。在分别产生IG[免疫球蛋白]G2b和IgG2a的细胞的DNA中发现涉及γ 2b和γ 2a CH [H链恒定区]基因的重排的证据。IgG 2b分泌系的DNA缺乏可检测的C γ 1基因，而IgG 2a分泌系的DNA缺乏可检测的C γ 1和C γ 2b基因。分泌伊加的细胞系的DNA缺乏可检测的C γ。基因.这些观察结果涉及删除的H链开关的机制，并允许在小鼠基因组中的一些CH基因的初步排序。
The arrangement of the genes for the mouse H chain subclasses .gamma.2b, and .gamma.2a and .gamma.1 was examined by the Southern hybridization procedure. Evidence was found for rearrangement involving the .gamma.2b and .gamma.2a CH [H chain constant region] genes in the DNA of cells making Ig[immunoglobulin]G2b and IgG2a, respectively. The DNA of an IgG2b-secreting line lacks detectable C.gamma.1 gene, while the DNA of an IgG2a-secreting line lacks detectable C.gamma.1 and C.gamma.2b genes. The DNA of a cell line secreting IgA lacks detectable C.gamma. genes. These observations implicate deletion in the mechanism of the H-chain switch and allow a preliminary ordering of some of the CH genes in the mouse genome.