Detection and Characterization of Low Abundance Glycopeptides Via Higher-Energy C-Trap Dissociation and Orbitrap Mass Analysis
Detection and Characterization of Low Abundance Glycopeptides Via Higher-Energy C-Trap Dissociation and Orbitrap Mass Analysis
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DOI：
10.1007/s13361-011-0273-y
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发表时间：
2012-01-01
影响因子：
3.2
通讯作者：
Raftery, Mark J.
中科院分区：
文献类型：
--
作者：
Hart-Smith, Gene;Raftery, Mark J.
糖蛋白组学固有的相当复杂的特性限制了糖肽的大规模质谱学分析，而相关技术仍在积极开发中以解决相关的分析困难。在这里，我们应用Orbitrap质量分析和高能C-陷阱解离(HCD)来促进对低丰度糖肽复杂混合物的组成和异质性的详细了解。通过在质量测量误差时产生诊断氧产物离子
Broad-scale mass spectrometric analyses of glycopeptides are constrained by the considerable complexity inherent to glycoproteomics, and techniques are still being actively developed to address the associated analytical difficulties. Here we apply Orbitrap mass analysis and higher-energy C-trap dissociation (HCD) to facilitate detailed insights into the compositions and heterogeneity of complex mixtures of low abundance glycopeptides. By generating diagnostic oxonium product ions at mass measurement errors of