Silencing Sl-EBF1 and Sl-EBF2 expression causes constitutive ethylene response phenotype, accelerated plant senescence, and fruit ripening in tomato.
Silencing Sl-EBF1 and Sl-EBF2 expression causes constitutive ethylene response phenotype, accelerated plant senescence, and fruit ripening in tomato.
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DOI：
10.1093/jxb/erp332
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发表时间：
2010-03
影响因子：
6.9
通讯作者：
Yingwu Yang;Yuehua Wu;Julien Pirrello;F. Regad;M. Bouzayen;W. Deng;Zheng-guo Li
中科院分区：
文献类型：
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作者：
Yingwu Yang;Yuehua Wu;Julien Pirrello;F. Regad;M. Bouzayen;W. Deng;Zheng-guo Li
乙烯是植物生长发育的重要激素，EBF（EIN 3-binding F-box）蛋白通过介导EIN 3/EIL蛋白的降解对乙烯信号通路起负调控作用。本研究报告的两个番茄F-box基因，Sl-EBF 1和Sl-EBF 2的EBF亚家族的鉴定。这两个基因在生殖和营养组织中以及在乙烯和生长素处理中显示出截然不同的表达模式。Sl-EBF 1和Sl-EBF 2基因在生殖器官发育的关键阶段受到积极调控。它们在花中的动态表达在花蕾到开花期和开花期到开花后的过渡期间，以及在果实成熟开始时，表明它们在乙烯需要刺激花开放和触发果实成熟的情况下的作用。VIGS介导的单个番茄EBF基因的沉默揭示了一种补偿机制，该机制倾向于通过增强第二S1-EBF基因的表达来维持S1-EBF表达的阈值水平。与这种补偿一致，沉默任一Sl-EBF基因的番茄植物与对照植物无法区分，表明Sl-EBF基因之间的功能冗余。相比之下，两种S1-EBF的共沉默导致乙烯相关表型。虽然EBF基因的报告，迄今为止，集中在他们的作用，在拟南芥中调节乙烯反应，本研究揭示了他们的作用，在调节番茄花和果实发育的关键阶段。这些数据支持这样的假设，即通过泛素/26 S蛋白酶体途径的蛋白质降解是果实成熟的控制点，并为工程果实品质开辟了新的线索。
The hormone ethylene regulates a wide range of plant developmental processes and EBF (EIN3-binding F-box) proteins were shown to negatively regulate the ethylene signalling pathway via mediating the degradation of EIN3/EIL proteins. The present study reports on the identification of two tomato F-box genes, Sl-EBF1 and Sl-EBF2 from the EBF subfamily. The two genes display contrasting expression patterns in reproductive and vegetative tissues and in response to ethylene and auxin treatment. Sl-EBF1 and Sl-EBF2 genes are actively regulated at crucial stages in the development of the reproductive organs. Their dynamic expression in flowers during bud-to-anthesis and anthesis-to-post-anthesis transitions, and at the onset of fruit ripening, suggests their role in situations where ethylene is required for stimulating flower opening and triggering fruit ripening. VIGS-mediated silencing of a single tomato EBF gene uncovered a compensation mechanism that tends to maintain a threshold level of Sl-EBF expression via enhancing the expression of the second Sl-EBF gene. In line with this compensation, tomato plants silenced for either of the Sl-EBF genes were indistinguishable from control plants, indicating functional redundancy among Sl-EBF genes. By contrast, co-silencing of both Sl-EBFs resulted in ethylene-associated phenotypes. While reports on EBF genes to date have focused on their role in modulating ethylene responses in Arabidopsis, the present study uncovered their role in regulating crucial stages of flower and fruit development in tomato. The data support the hypothesis that protein degradation via the ubiquitin/26S proteasome pathway is a control point of fruit ripening and open new leads for engineering fruit quality.