HflB gene-based phytopathogenic classification of 'Candidatus phytoplasma mali' strains and evidence that strain composition determines virulence in multiply infected apple trees.
HflB gene-based phytopathogenic classification of 'Candidatus phytoplasma mali' strains and evidence that strain composition determines virulence in multiply infected apple trees.
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基于 HflB 基因的“Candidatus phytoplasma mali”菌株的植物病原分类以及菌株组成决定多重感染苹果树毒力的证据。
DOI：
10.1094/mpmi-05-11-0126
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
B. Schneider
中科院分区：
文献类型：
--
作者：
E. Seemüller;M. Kampmann;Emese Kiss;B. Schneider
通过对来自27株症状差异较大的苹果树上的‘假丝酵母菌’的病理学和分子生物学数据的分析，对感染苹果增殖植原体的菌株进行了分子特征分析和分类。单链构象多态性分析和ATP00464型hflB基因可变区的序列分析表明，这些来源是由数量相近的单菌株和多菌株组成的。后者由2至5个不同的菌株组成。对轻度和重度单一菌株材料的克隆序列分析导致了两组阅读片段，根据它们的毒力，它们在系统图上聚在了遥远的地方。基于这些数据，多菌株序列的聚类图表明，几乎所有的菌株都是由轻度和重度菌株组成的。代表轻度和重度毒株的序列的明显聚集性与核苷酸和氨基酸水平上的一系列分子标记有关。数据表明，多品系材料的毒力取决于出现的弱菌株和严重菌株的比例，因为温和材料的特点是代表弱菌株的序列占优势，反之亦然。有证据表明，可能会发生种群变化和其他事件，从而极大地改变多品系种质的毒力。
Analysis of pathological and molecular data of 'Candidatus Phytoplasma mali' accessions from 27 apple trees differing considerably in symptomatology was used to molecularly characterize and classify strains of the infecting apple proliferation phytoplasma. Single-strand conformation polymorphism and sequence analysis of a variable fragment of ATP00464-type hflB gene revealed that these sources consisted of single-strain and multiple-strain accessions that occurred in similar numbers. The latter group was composed of two to five distinct strains. Analysis of cloned sequences of mild and severe single-strain accessions resulted in two groups of reads that clustered, according to their virulence, distantly in the phylogram. Based on this data, the clustering patterns of multiple-strain accession sequences indicated that nearly all of them were composed of mild and severe strains. The distinct clustering of sequences representing mild and severe strains was associated with a range of molecular markers at the nucleotide and amino acid level. Data indicate that the virulence of multiple-strain accessions is determined by the ratio of the occurring mild and severe strains in that mild accessions were characterized by the predominance of sequences representing mild strains and vice versa. There is evidence that shifts in the population and other events may occur that drastically alter virulence of multiple-strain accessions.