Crystallization and preliminary X-ray crystallographic studies of the axin DIX domain.
Crystallization and preliminary X-ray crystallographic studies of the axin DIX domain.
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DOI：
10.1107/s1744309107022579
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发表时间：
2007-06
期刊：
影响因子：
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通讯作者：
N. Shibata;Y. Tomimoto;T. Hanamura;R. Yamamoto;M. Ueda;Y. Ueda;N. Mizuno;H. Ogata;H. Komori;Y. Shomura;M. Kataoka;S. Shimizu;J. Kondo;Hideki Yamamoto;A. Kikuchi;Y. Higuchi
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文献类型：
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作者：
N. Shibata;Y. Tomimoto;T. Hanamura;R. Yamamoto;M. Ueda;Y. Ueda;N. Mizuno;H. Ogata;H. Komori;Y. Shomura;M. Kataoka;S. Shimizu;J. Kondo;Hideki Yamamoto;A. Kikuchi;Y. Higuchi
Axin是经典Wnt信号通路的负调节因子，其介导糖原合成酶激酶3 β对β-连环蛋白的磷酸化。DIX结构域的大鼠轴，这是重要的同源寡聚化和与其他监管机构在Wnt途径的相互作用，纯化和结晶坐滴气相扩散技术使用聚乙二醇6000和硫酸锂作为结晶剂。晶体属P6（1）或P6（5）空间群，晶胞参数a = B = 91.49，c = 84.92。已收集到标称分辨率为2.9 A的X射线衍射数据集。
Axin is a negative regulator of the canonical Wnt signalling pathway that mediates the phosphorylation of beta-catenin by glycogen synthase kinase 3beta. The DIX domain of rat axin, which is important for its homooligomerization and interactions with other regulators in the Wnt pathway, was purified and crystallized by the sitting-drop vapour-diffusion technique using polyethylene glycol 6000 and lithium sulfate as crystallization agents. Crystals belong to space group P6(1) or P6(5), with unit-cell parameters a = b = 91.49, c = 84.92 A. An X-ray diffraction data set has been collected to a nominal resolution of 2.9 A.