REGIONAL AND TEMPORAL GLYCEROL CHANGES INDUCED BY FOREBRAIN ISCHEMIA IN GERBILS
REGIONAL AND TEMPORAL GLYCEROL CHANGES INDUCED BY FOREBRAIN ISCHEMIA IN GERBILS
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DOI：
10.1016/0304-3940(92)90809-l
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发表时间：
1992-12-14
影响因子：
2.5
通讯作者：
SPATZ, M
中科院分区：
文献类型：
--
作者：
BERTRAND, N;ISHII, H;SPATZ, M
研究了沙鼠双侧颈动脉单独闭塞15 min或不同循环周期时纹状体、海马和皮层中甘油和能量代谢物的颞缺血变化。同一组织样品在高氯酸萃取后，用简单的酶促荧光光谱法测定甘油和能量储备。脑缺血使甘油水平升高（8- 10倍）并耗尽能量储存。在再循环的第一个小时内，甘油含量下降，此后（2小时）在所有结构中趋于正常化。然而，纹状体和海马体中的甘油含量仍然是各自对照的两倍。同时，在这些结构中观察到能量储备的不完全恢复。结果表明，甘油不是一个稳定的缺血后膜损伤指标。
Temporal ischemic changes in glycerol and energy metabolites were studied in the striatum, hippocampus and cortex of gerbils subjected to 15 min of bilateral carotid artery occlusion alone or with various periods of recirculation. The same tissue sample served for the determination of glycerol and energy reserve by a simple enzymatic fluoro- and spectrometric assay after perchloric acid extraction. Cerebral ischemia increased the levels of glycerol (8- to 10-fold) and depleted the energy stores. During the first hour of recirculation, the glycerol content decreased and thereafter (at 2 h), normalized in all structures. However, the glycerol content was still twice as high in the striatum and hippocampus as compared to their respective controls. At the same time, an incomplete restoration of energy reserves was observed in these structures. The findings indicate that glycerol is not a stable postischemic indicator of the ischemia-induced membrane damage.