Postnatal differentiation and regional histological variations in the ductus epididymidis of the Congjiang Xiang pig
Postnatal differentiation and regional histological variations in the ductus epididymidis of the Congjiang Xiang pig
复制标题
从江湘猪附睾管的产后分化及区域组织学变化
DOI：
10.1016/j.tice.2020.101411
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发表时间：
2020-12-01
期刊：
影响因子：
2.6
通讯作者：
Yang, Yi
中科院分区：
文献类型：
--
作者：
Meng, Li-Jie;Wang, Wei-Yong;Yang, Yi
从江香猪是我国稀有的小型猪品种，其附睾在出生后发育过程中的组织学特征尚不清楚。为明确从江香猪附睾各部位在生后发育过程中的组织形态学差异，对24头从江香猪从初生（15 d）、初情期（30 d）、初情期（60 d）至成年（180 d）的附睾进行了组织形态学观察。附睾不同区域（I-V）的生后分化从出生开始，一直持续到成熟，表现出区域性差异。发育进展为椎间盘近端。在新生儿期，沃尔夫管分化开始于远端区域，然后上升到中间区域，分别形成V区、IV区和III区。从新生期到青春期后期，上皮细胞逐渐分化为假复层柱状细胞，具有静纤毛。青春期后，上皮细胞发生重排，分化加快，管腔内可见精子，尤以II区管腔为甚。在III区，经常观察到晕和顶细胞。在青春期后阶段，经常观察到透明细胞在区域IV-V，和附睾管的大小显着增加，完全挤满了精子。本研究为今后从江香猪繁殖力的评价提供了依据。青春期后，上皮细胞发生重排，分化加快，管腔内可见精子，尤以II区管腔为甚。在III区，经常观察到晕和顶细胞。在青春期后阶段，经常观察到透明细胞在区域IV-V，和附睾管的大小显着增加，完全挤满了精子。本研究为今后从江香猪繁殖力的评价提供了依据。
The Congjiang Xiang pig is a rare Chinese miniature breed whose epididymal histologic features through the postnatal development are poorly understood. To clarify the histomorphological differences between each region of epididymis during postnatal development, 24 male Congjiang Xiang pigs aged from neonatal (15 d), per-puberty (30 d), puberty (60 d) to adult (180 d) stages, were examined. Postnatal differentiation of the different regions (I-V) of the epididymis started from birth and continued until maturity that showed regional variations. Developmental progression was disco-proximal. At the neonatal stage, Wolffian duct differentiation starts in the distal region, then ascends to the middle region which forms regions V, IV and III, respectively. A simple lined cuboidal in the epidydimal epithelial, which gradually differentiated into a pseudostratified columnar with stereocilia from neonatal to post-pubertal. After puberty cell rearrangement occurred in the epithelium, differentiation accelerated, and spermatozoon seen in the lumen, especially the lumen of region II. In region III, both halo and apical cells were frequently observed. At the post-pubertal stage, clear cells were frequently observed in Region IV-V, and the epididymal duct was markedly increased in size and fully packed with spermatozoa. The information presented in this study will be helpful for future evaluations of Congjiang Xiang pig fertility. After puberty cell rearrangement occurred in the epithelium, differentiation accelerated, and spermatozoon seen in the lumen, especially the lumen of region II. In region III, both halo and apical cells were frequently observed. At the post-pubertal stage, clear cells were frequently observed in Region IV-V, and the epididymal duct was markedly increased in size and fully packed with spermatozoa. The information presented in this study will be helpful for future evaluations of Congjiang Xiang pig fertility.