Loss of GATA1 and gain of FLI1 expression during thrombocyte maturation.
Loss of GATA1 and gain of FLI1 expression during thrombocyte maturation.
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DOI：
10.1016/j.bcmd.2009.12.012
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发表时间：
2010-03-15
影响因子：
2.3
通讯作者：
Kim, Seongcheol
中科院分区：
文献类型：
--
作者：
Jagadeeswaran, Pudur;Lin, Shuo;Weinstein, Brant;Hutson, Angela;Kim, Seongcheol
本研究在斑马鱼转基因系G1-GM2和TG(FLI1:EGFP)y1的血栓细胞中表达了GATA1和FLI1基因启动子驱动GFP的基因。我们在G1-GM2和TG(fli1:EGFP)y1系中发现了两个离散的血小板群体，它们被GFP标记为：(1)GFP+血小板的标记越强烈，(2)GFP+血小板的标记越不强烈。G1-GM2系GFP+标记越强烈的血小板和TG(fli1:EGFP)y1系GFP+标记越不强烈的血小板对应于年轻的血小板。这些结果表明，年轻血栓细胞具有较高的GATA1启动子活性，而成熟血栓细胞具有更多的FLI1基因启动子转录。这一发现表明，随着血小板的成熟，GATA1的逐渐丧失和FLI1的表达增加，并且FLI1的过表达可能有助于维持血小板谱系。此外，与巨核细胞中发现的转录因子相似的存在提出了脊椎动物血小板可能是哺乳动物巨核细胞的前身的可能性，因此可以作为研究巨核细胞成熟的模型。
In this paper we characterized expression of GATA1 and FLI1 gene promoters in thrombocytes of zebrafish transgenic lines, G1-GM2 and TG(fli1:EGFP)y1 that carry transgenes of GATA1 and FLI1 gene promoters driving GFP. We found two discrete populations of thrombocytes verified by morphology, labeled with GFP in both G1-GM2 and TG(fli1:EGFP)y1 lines: (1) the more intensely labeled GFP+ thrombocyte, and (2) the less intensely labeled GFP+ thrombocytes. The more intensely labeled GFP+ thrombocyte in G1-GM2 line and the less intensely labeled GFP+ thrombocytes in the TG(fli1:EGFP)y1 line corresponded to young thrombocytes. These results showed that young thrombocytes have higher GATA1 promoter activity, while mature thrombocytes have more FLI1 gene promoter transcription. This finding suggests that there is a gradual loss of GATA1 and gain of FLI1 expression as the thrombocytes mature, and this overexpression of FLI1 may help maintain the thrombocyte lineage. Furthermore, the presence of transcriptional factors similar to those found in megakaryocytes raises the possibility that vertebrate thrombocytes may be the forerunners of mammalian megakaryocytes and, therefore, could serve as a model to study megakaryocyte maturation.
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