The origin of some acid hydrolases of the fluid of the rat cauda epididymidis.
The origin of some acid hydrolases of the fluid of the rat cauda epididymidis.
复制标题
大鼠附睾尾液中某些酸性水解酶的来源。
作者：
Luis S. Mayorga;Francisco Bertini
N-乙酰-β-D-氨基葡萄糖苷酶的活性（NAG，60.1单位/mg蛋白质）和酸性磷酸酶（57.7单位/mg蛋白），在没有睾丸任何贡献的情况下，（从灌注和分离的附睾尾或从相应的输出管结扎40天的附睾中获得的液体）显著高于正常体液中这些酶的活性（分别为39.6和41.2单位/mg蛋白）。局部形成的液体的芳基硫酸酯酶活性（11.2单位/mg蛋白质）低于正常液体（74.1单位/mg蛋白质）。睾丸网液中芳基硫酸酯酶相对丰富，因为芳基硫酸酯酶与酸性磷酸酶活性的比率在该液中比在局部形成的液中高17倍。结果表明，正常附睾液中NAG和酸性磷酸酶的活性来源于附睾组织，而芳基硫酸酯酶的活性主要来源于睾丸。
The activity of N-acetyl-beta-D-glucosaminidase (NAG, 60.1 units/mg protein) and of acid phosphatase (57.7 units/mg protein) in fluid from the cauda epididymidis formed without any contribution from the testis (fluid obtained from a perfused and isolated cauda epididymidis or from an epididymis whose corresponding efferent ducts had been ligated for 40 days) was significantly higher than the activity of these enzymes in normal fluid (39.6 and 41.2 units/mg protein, respectively). Arylsulphatase activity of the locally formed fluid (11.2 units/mg protein) was lower than that of normal fluid (74.1 units/mg protein). The rete testis fluid was relatively rich in arylsulphatase since the ratio of arylsulphatase to acid phosphatase activity was 17 times higher in this fluid than in locally formed fluid. It is concluded that the activities of NAG and acid phosphatase in normal fluid from the epididymis originate in the epididymal tissue, while most of the arylsulphatase activity comes from the testis.