ACTIVATION OF LYMPHOCYTES-T BY LECTINS AND CARBOHYDRATE-OXIDIZING REAGENTS VIEWED AS AN IMMUNOLOGICAL RECOGNITION OF CELL-SURFACE MODIFICATIONS SEEN IN THE CONTEXT OF SELF MAJOR HISTOCOMPATIBILITY COMPLEX ANTIGENS
ACTIVATION OF LYMPHOCYTES-T BY LECTINS AND CARBOHYDRATE-OXIDIZING REAGENTS VIEWED AS AN IMMUNOLOGICAL RECOGNITION OF CELL-SURFACE MODIFICATIONS SEEN IN THE CONTEXT OF SELF MAJOR HISTOCOMPATIBILITY COMPLEX ANTIGENS
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DOI：
10.1002/eji.1830110607
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发表时间：
1981-01-01
影响因子：
5.4
通讯作者：
ERSSON, B
中科院分区：
文献类型：
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作者：
KIMURA, A;ERSSON, B
研究了有丝分裂物质对T细胞的多克隆激活是通过有丝分裂原受体对T细胞进行真正的抗原非依赖性刺激的结果，绕过了T细胞特异性识别的要求，还是一种与抗原诱导的T细胞激活在原理和要求上相似的免疫反应。有几条证据有力地支持了后一种解释。几种有丝分裂物质诱导多克隆T细胞活化的能力主要依赖于主要组织相容性复合体（MHC）产物的细胞表面表达。因此，针对I类（SD）决定因子或II类（Ia）产物的同种抗血清或单克隆抗体在应答细胞群上的存在导致对有丝分裂反应的相当大的剂量依赖性抑制。使用正常F1小鼠或F1 .fwdarw的脾脏细胞进行的其他研究。亲本骨髓嵌合体为MHC产物在有丝分裂T细胞活化中的特异性免疫作用提供了最直接和最令人信服的证据。在这里，正常F1 T细胞对concanavin A的反应被同种异体抗血清抑制。h -2杂合F1 .fwdarw。亲本嵌合体仅被抗亲本H-2的抗血清抑制，亲本嵌合体在其中成熟并具有功能性的mhc限制性辅助T细胞。根据在这些有丝分裂反应中观察到的对MHC表达的依赖性，专门检查了赋予某些凝集素发挥有丝分裂作用的固有特征和反应性。使用一组20种不同的凝集素作为细胞表面糖蛋白的亲和力吸收剂，只有有丝分裂凝集素能够结合细胞表面表达的H-2产物（K/D和Ia）。所获得的结果进行了讨论，以支持这一概念，即丝裂原诱导的T细胞反应涉及在自身H-2抗原的背景下对细胞表面修饰的免疫识别。
Whether polyclonal activation of T cells by mitogenic substances results as a consequence of a truly antigen-independent stimulation of T cells through mitogen receptors, bypassing requirements for T cell-specific recognition, or an immunological reaction, similar in principle and requirements to T cell activation induced by antigen, was studied. Several lines of evidence provided strong support for the latter interpretation. The ability of several mitogenic substances to induce polyclonal T cell activation was crucially dependent upon cell surface expression of major histocompatibility complex (MHC) products. Thus, the presence of alloantisera or monoclonal antibodies directed against either class I (SD) determinants or class II (Ia) products on the responding cell population resulted in a sizeable dose-dependent inhibition of the mitogenic response. Additional studies using spleen cells from normal F1 mice or F1 .fwdarw. parent bone marrow chimeras provided the most direct and convincing evidence for a specific immunological role of MHC products in mitogenic T cell activation. Here, the concanavalin A response of normal F1 T cells was inhibited by alloantisera against either parental haplotype; the H-2-heterozygous F1 .fwdarw. parent chimeras were only inhibited by antisera against the H-2 of the parent in which they matured and possessed functional, MHC-restricted helper T cells. The inherent characteristics and reactivities which endow only certain lectins to exert a mitogenic effect were specifically examined in light of the observed dependence for MHC expression in these mitogenic reactions. Using a panel of 20 different lectins as affinity absorbents for cell surface glycoproteins, only mitogenic lectins were capable of binding to cell surface-expressed H-2 products (both K/D and Ia). The obtained results are discussed in support of the concept that mitogen-induced T cell responses involve an immunological recognition of cell surface modifications seen in the context of self H-2 antigens.