Environmental Factors and the Symbiont Cardinium Influence the Bacterial Microbiome of Spider Mites Across the Landscape
Environmental Factors and the Symbiont Cardinium Influence the Bacterial Microbiome of Spider Mites Across the Landscape
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DOI：
10.1007/s00248-023-02314-7
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发表时间：
2024-12-01
影响因子：
3.6
通讯作者：
Bing，Xiao-Li
中科院分区：
文献类型：
--
作者：
Liu，Huan-Huan;Chen，Lei;Bing，Xiao-Li
微生物在节肢动物的生物学、生态学和进化中起着关键作用。尽管积累了关于节肢动物中微生物群落的数据，这些节肢动物使用刺吸式口器以植物为食，但我们仍然缺乏对微生物群的组成和组装因素的全面了解，特别是在实地收集的蜘蛛螨中。本文应用16SrRNA扩增子序列分析技术，对采自中国26个地点的8种叶螨（阿卡里：叶螨科）共140个样本420个个体的细菌群落特征进行了研究。结果表明，不同种类、不同地点和不同植物的叶螨的细菌组成存在显著差异。不同环境对叶螨细菌群落的相对贡献不同。纬度和降水量是影响细菌群落组成的主要因素。细菌群落的多样性与螨类分布的地理距离呈显著相关。蜘蛛螨细菌群落的组装似乎主要受随机过程的影响。此外，共生Cardinium被发现在形成许多Tetranychus物种的微生物群中很重要。镉的相对丰度在维也纳毛癣菌、毛癣菌、毛癣菌中均大于50%。urticaeG，T. urticaeR和T.土耳其斯坦。从我们的分析RemovingCardiniumreads显着改变Shannon多样性指数和加权β多样性在这些species.All，这项研究提供了新的见解细菌多样性模式，有助于我们的知识节肢动物和他们的细菌群落之间的共生关系。
Microbes play a key role in the biology, ecology, and evolution of arthropods. Despite accumulating data on microbial communities in arthropods that feed on plants using piercing-sucking mouthparts, we still lack a comprehensive understanding of the composition and assembly factors of the microbiota, particularly in field-collected spider mites. Here, we applied 16S rRNA amplicon sequencing to investigate the characters of the bacterial community in 140 samples representing 420 mite individuals, belonging to eightTetranychusspecies (Acari: Tetranychidae) collected from 26 sites in China. The results showed that the bacterial composition of spider mites varied significantly among different species, locations, and plants. The environment showed a significant influence on the bacterial community of spider mites, with different relative contributions. Latitude and precipitation were found to be the main factors influencing the bacterial community composition. The dissimilarity of bacterial community and geographical distance between mite locations were significantly correlated. The assembly of spider mite bacterial communities seemed to be mainly influenced by stochastic processes. Furthermore, the symbiontCardiniumwas found to be important in shaping the microbiota of manyTetranychusspecies. The relative abundance ofCardiniumwas > 50% inT. viennensis,T. urticaeG,T. urticaeR, andT. turkestani. RemovingCardiniumreads from our analysis significantly changed Shannon diversity index and weighted beta diversity in these species.Altogether, this study provides novel insights into bacterial diversity patterns that contribute to our knowledge of the symbiotic relationships between arthropods and their bacterial communities.