Decolorization and biosorption for Congo red by system rice hull Schizophyllum sp F17 under solid-state condition in a continuous flow packed-bed bioreactor
Decolorization and biosorption for Congo red by system rice hull Schizophyllum sp F17 under solid-state condition in a continuous flow packed-bed bioreactor
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DOI：
10.1016/j.biortech.2008.01.049
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发表时间：
2008-10-01
影响因子：
11.4
通讯作者：
Jia, Rong
中科院分区：
文献类型：
--
作者：
Li, Xudong;Jia, Rong
合成染料是来自各个行业的重要化学污染物。以稻壳-裂褶菌F17为原料，在固定床生物反应器中建立了一种高效、简单的固态连续脱色系统，用于刚果红的脱色。在该脱色体系中，存在两种脱色机理，一种是裂褶菌F17的脱色作用，另一种是稻壳船体的生物吸附作用。染料浓度和水力停留时间对脱色效果有较大影响，采用响应面法对染料浓度和水力停留时间进行了定量分析和优化。采用2（2）完全析因中心组合设计（CCD），建立了染料浓度和水力停留时间对总脱色率（R-2 = 0.902）、裂褶菌F17脱色率（R-2 = 0.866）和稻壳船体吸附率（R-2 = 0.890）影响的3个二阶多项式模型。响应面等高线图构建，以显示染料浓度和HRT的个体和累积效应，以及最佳值。当染料浓度为142.63 mg/L，水力停留时间为41 h时，裂褶菌F17的总脱色率最高，为89.71%;当染料浓度为110.7 mg/L，水力停留时间为29.4 h时，裂褶菌F17的总脱色率最高，为60.44%。同时，研究了锰过氧化物酶（MnP）在脱色过程中的作用。本研究证明了白腐真菌在固态发酵生物反应器中对合成染料进行连续脱色的可行性。(C)2008爱思唯尔有限公司保留所有权利。
Synthetic dyes are important chemical pollutants from various industries. This work developed an efficient and relatively simple continuous decolorization system rice hull-Schizophyllum sp. F17 under solid-state condition in a packed-bed bioreactor, for decolorizing Congo red. In the decolorization system, two decolorization mechanisms exist, one is decolorization by Schizophyllum sp. F17, the other is biosorption by rice hull. The decolorization efficiency was greatly affected by dye concentration and hydraulic retention time (HRT), which were quantificationally analyzed and optimized through response surface methodology (RSM). A 2(2) full factorial central composite design (CCD) was performed, and three second order polynomial models were generated to describe the effects of dye concentration and HRT on total decolorization (R-2 = 0.902), decolorization by Schizophyllum sp. F17 (R-2 = 0.866) and biosorption by rice hull (R-2 = 0.890). Response surface contour plots were constructed to show the individual and cumulative effects of dye concentration and HRT, and the optimum values. A maximum total decolorization 89.71% and maximum decolorization by Schizophyllum sp. F17 60.44% was achieved at dye concentration 142.63 mg/L, HRT41 h, and dye concentration 110.7 mg/L, HRT 29.4 h, respectively. Meanwhile, the role of manganese peroxidase (MnP) in the decolorizaion process was investigated. This study proved the feasibility of continuous mode for decolorizing synthetic dyes by white-rot fungi in solid-state fermentation bioreactors. (C) 2008 Elsevier Ltd. All rights reserved.