Photo cross-linked biodegradable hydrogels for enhanced vancomycin loading and sustained release
Photo cross-linked biodegradable hydrogels for enhanced vancomycin loading and sustained release
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DOI：
10.1007/s10118-013-1358-9
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发表时间：
2013-11
影响因子：
4.3
通讯作者：
Jingzhe Zhang;Chunsheng Xiao;Jincheng Wang;Xiu-li Zhuang;Xuesi Chen
中科院分区：
文献类型：
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作者：
Jingzhe Zhang;Chunsheng Xiao;Jincheng Wang;Xiu-li Zhuang;Xuesi Chen
制备了一系列基于葡聚糖和聚（L-谷氨酸）的生物可降解水凝胶，用于有效负载和释放万古霉素。在光引发剂I2959的存在下，通过光交联甲基丙烯酸葡聚糖和聚（L-谷氨酸）-g-甲基丙烯酸羟乙酯（PGH）来简单地实现水凝胶的制备。用FTIR和SEM对凝胶的结构进行了表征。水凝胶的溶胀和酶降解行为进行了研究，是依赖于在水凝胶中的聚（L-谷氨酸）的含量。凝胶中聚谷氨酸含量越高，溶胀率越高，降解速度越快。结果表明，PGH比越高的水凝胶，其载药率越高，这可能是由于水凝胶中羧酸基团与VCM的铵基之间存在静电相互作用的结果。载药水凝胶的体外释药实验表明，载药水凝胶对VCM的缓释作用可达72 h，而载药水凝胶的体外抗菌实验表明，载药水凝胶对甲氧西林具有良好的耐药性。金黄色葡萄球菌（MRSA）抑制延长至7天。这些结果表明，通过原位光交联形成的生物可降解水凝胶有望作为组织工程中抗菌药物局部释放的支架或涂层材料。
A series of biodegradable hydrogels based on dextran and poly(L-glutamic acid) were fabricated for effective vancomycin loading and release. The preparation of hydrogels was simply achieved by photo cross-linking of methacrylated dextran and poly(L-glutamic acid)-g-hydroxyethyl methacrylate (PGH) in the presence of photoinitiator I2959. The structures of hydrogels were characterized by FTIR and SEM. The swelling and enzymatic degradation behaviors of hydrogels were examined to be dependent on the poly(L-glutamic acid) content in the hydrogels. The higher content of poly(L-glutamic acid) in the gel, the higher swelling ratio and quicker degradation were observed. More interestingly, the hydrogel with higher PGH ratio showed higher vancomycin (VCM) loading content, which might be due to the electrostatic interaction between carboxylate groups in hydrogel and ammonium group of VCM.In vitrodrug release from the VCM-loaded hydrogels in aqueous solution exhibited sustained release of VCM up to 72 h, while thein vitroantibacterial test based on the VCM-loaded hydrogel showed an efficient Methicillin-ResistantS. aureus(MRSA) inhibition extending out to 7 days. These results demonstrated that the biodegradable hydrogels which formed byin situphoto-cross linking would be promising as scaffolds or coatings for local antibacterial drug release in tissue engineering.