A nitrile-mediated aptasensor for optical anti-interference detection of acetamiprid in apple juice by surface-enhanced Raman scattering
A nitrile-mediated aptasensor for optical anti-interference detection of acetamiprid in apple juice by surface-enhanced Raman scattering
复制标题
腈介导适体传感器表面增强拉曼散射光学抗干扰检测苹果汁中啶虫脒
DOI：
10.1016/j.bios.2019.111672
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发表时间：
2019-12-01
影响因子：
12.6
通讯作者：
Liu, Tingting
中科院分区：
文献类型：
--
作者：
Sun, Yue;Li, Zhihua;Liu, Tingting
目前，农药的检测是保证食品安全的关键，但由于复杂的食品基质中存在生物干扰物，农药的检测仍然具有挑战性。本研究建立了一种用于啶虫脒痕量检测的光学抗干扰表面增强拉曼散射（Sers）适体传感器系统。用含C=N键的4-（巯基甲基）苯甲腈（MMBN）作为拉曼标记，在拉曼静默光谱窗（1800.2800 cm（-1））内产生一个尖锐的拉曼峰（2227 cm（-1）），在此窗内绝大多数分子没有拉曼信号。以金纳米粒子（AuNPs）为拉曼探针，以银纳米粒子修饰的硅片（AgNPs@Si）为表面增强拉曼散射（Sers）基底，合成了以多聚腺嘌呤（polyA）为载体的金纳米粒子（MMBN-AuNPs-aptamer）。由于啶虫脒分子能与适体特异性联合收割机结合，阻止了MMBN-AuNPs-aptamer-cDNA-AgNPs@Si（表示为“Au-AgNPs@Si”）杂交体的形成，因此随着啶虫脒浓度的增加，AuAgNPs@Si中MMBN的拉曼信号强度降低。在最佳测定条件下，啶虫脒在25-250 nM范围内与峰面积呈良好的线性关系，检测限为6.8 nM。最后，研制的适体传感器成功用于苹果汁中啶虫脒含量的测定。因此，这种新型的抗干扰Sers适体传感器可能是一个有前途的啶虫脒传感器，用于食品安全保障。
Currently, the detection of pesticide is critical for food safety assurance, but it is still challenging due to the presence of biological interferents from complex food matrix. In this study, we developed an optical anti-interference surface-enhanced Raman scattering (SERS) aptasensor system for trace detection of acetamiprid. 4-(Mercaptomethyl) benzonitrile (MMBN) containing C=N bond was used as Raman tag to provide a sharp peak (2227 cm(-1)) in the Raman-silent spectral window (1800.2800 cm(-1)) where no Raman signal existed for most of molecules. Gold nanoparticles (AuNPs) bonded with polyadenine (polyA)-mediated aptamer and Raman tag (MMBN-AuNPs-aptamer) was synthesized as Raman probe, while the complementary DNA (cDNA) conjugated with AgNPs-decorated silicon wafer (AgNPs@Si) was used as SERS substrate. As acetamiprid molecule could specifically combine with aptamer, preventing the formation of MMBN-AuNPs-aptamer-cDNA-AgNPs@Si (expressed as "Au-AgNPs@Si") hybrid through DNA sequence linking, Raman signal intensities of MMBN in AuAgNPs@Si decreased when the concentration of acetamiprid increased. Under the optimum assay condition, the proposed method displayed a linear response for acetamiprid detection in the range of 25-250 nM with a low detection limit of 6.8 nM. Finally, the developed aptasensor was successfully used to determine acetamiprid content in apple juice. Accordingly, this novel anti-interference SERS aptasensor could be a promising acetamiprid sensor for food safety assurance.