Cyclooxygenase inhibition does not impact the pressor response during static or dynamic mechanoreflex activation in healthy decerebrate rats.
Cyclooxygenase inhibition does not impact the pressor response during static or dynamic mechanoreflex activation in healthy decerebrate rats.
复制标题
环加氧酶抑制不会影响健康去大脑大鼠静态或动态机械感受反射激活期间的升压反应。
DOI：
10.1152/ajpregu.00080.2019
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Copp,StevenW
中科院分区：
文献类型：
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作者：
Rollins,KorynneS;Hopkins,TylerD;Butenas,AlecL;Felice,KennedyP;Ade,CarlJ;Copp,StevenW
人类和动物的被动肢体运动和肢体肌肉拉伸是用于研究独立于收缩诱导的代谢产物产生的肌肉机械反射激活的常见实验策略。然而，环氧合酶（考克斯）代谢物是由骨骼肌牵拉在体外产生的，并已发现影响各种模型的机械反射激活。考克斯代谢产物是否影响去大脑大鼠小腿三头肌牵张性机械反射模型尚不清楚。我们研究了大鼠小腿三头肌肌肉拉伸对考克斯代谢产物前列腺素E2（PGE 2）的间质浓度的影响。腓肠三头肌牵拉后4 min，肌间质PGE 2浓度均高于基线值（增加38%，P= 0.01）和动态（增加56%，P< 0.01）（n= 10）。需要4分钟的方案来收集足够的微透析液用于PGE 2检测。体内骨骼肌拉伸能够产生考克斯代谢物的发现提示了以下假设：动脉内施用考克斯抑制剂吲哚美辛（1 mg/kg）将减少在静态和动态大鼠小腿三头肌拉伸的30 s（大鼠机械反射模型中最常用的持续时间）期间诱发的升压和心脏加速器反应。结果发现，吲哚美辛对30 s静态或动态牵张时的升压反应和心脏加速反应均无影响（P> 0.05，n = 9）。我们的结论是，尽管考克斯代谢物浓度增加的可能性，考克斯代谢物不激活或敏化细纤维肌肉传入刺激在30 s的静态或动态后肢骨骼肌拉伸在健康大鼠。
Passive limb movement and limb muscle stretch in humans and animals are common experimental strategies used to investigate activation of the muscle mechanoreflex independent of contraction-induced metabolite production. Cyclooxygenase (COX) metabolites, however, are produced by skeletal muscle stretch in vitro and have been found to impact various models of mechanoreflex activation. Whether COX metabolites influence the decerebrate rat triceps surae muscle stretch mechanoreflex model remains unknown. We examined the effect of rat triceps surae muscle stretch on the interstitial concentration of the COX metabolite prostaglandin E2(PGE2). Interstitial PGE2concentration was increased above baseline values by 4 min of both static (38% increase,P= 0.01) and dynamic (56% increase,P< 0.01) triceps surae muscle stretch (n= 10). The 4-min protocol was required to collect enough microdialysis fluid for PGE2detection. The finding that skeletal muscle stretch in vivo was capable of producing COX metabolites prompted the hypothesis that intra-arterial administration of the COX inhibitor indomethacin (1 mg/kg) would reduce the pressor and cardioaccelerator responses evoked during 30 s (the duration most commonly used in the rat mechanoreflex model) of static and dynamic rat triceps surae muscle stretch. We found that indomethacin had no effect (P> 0.05,n= 9) on the pressor or cardioaccelerator response during 30 s of either static or dynamic stretch. We conclude that, despite the possibility of increased COX metabolite concentration, COX metabolites do not activate or sensitize thin-fiber muscle afferents stimulated during 30 s of static or dynamic hindlimb skeletal muscle stretch in healthy rats.