Association study between C7673T polymorphism in apolipoprotein B gene and cerebral infarction with family history in a Chinese population.
Association study between C7673T polymorphism in apolipoprotein B gene and cerebral infarction with family history in a Chinese population.
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DOI：
10.4103/0028-3886.57805
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发表时间：
2009-09
期刊：
影响因子：
2.7
通讯作者：
Le Zhang;Yi Zeng;Ming-ming Ma;Qi-dong Yang;Zhong-yang Hu;Xiao-ping Du
中科院分区：
文献类型：
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作者：
Le Zhang;Yi Zeng;Ming-ming Ma;Qi-dong Yang;Zhong-yang Hu;Xiao-ping Du
载脂蛋白B（ApoB）水平已被证明与缺血性卒中的风险有关。载脂蛋白B基因（APOB）多态性可能影响ApoB水平和缺血性脑卒中的风险，但其是否与有家族史的缺血性脑梗死（CI）的风险有关尚不清楚。目的探讨载脂蛋白B基因C7673 T多态性与长沙地区汉族人群慢性乙型肝炎的相关性。研究人群包括47例CIFH患者和83例无家族史脑梗死（CINFH）患者。对照人群包括100例年龄、性别和种族背景匹配的无缺血性卒中病史的健康受试者。材料与方法采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法分析APOB C7673 T多态性。还通过标准酶法和酶联免疫吸附试验（ELISA）检查血浆脂质水平。采用SPSS14.0统计软件包进行统计学分析。P值< 0.05视为显著。结果APOB基因C7673 T多态性与脑梗死，尤其是长沙地区汉族人群的CIFH相关。C7673 T多态性T等位基因频率在CIFH组和CINFH组均显著高于对照组（P < 0.01; P < 0.05）。CIFH组T等位基因频率（0.17）高于CINFH组（0.09）（P = 0.056）。在CINFH组和CINFH组中，TT和TC基因型的血清TC和LDL胆固醇水平显著高于CC基因型（P < 0.01），而HDL胆固醇水平显著低于CC基因型（P < 0.05）。结论APOB基因C7673 T多态性与脑梗死，尤其是CIFH的发生有关，可能与血脂水平的改变有关。
BACKGROUND Apolipoprotein B (ApoB) levels have been shown to be associate with risk of ischemic stroke. The apolipoprotein B gene (APOB) polymorphisms may influence levels of ApoB and risk of ischemic stroke, but whether they are associated with risk of ischemic cerebral infarction (CI) with family history (CIFH) or not is unknown. AIMS To investigate the possible association of the polymorphism of APOB C7673T with CIFH in Han Chinese from Changsha. SETTINGS AND DESIGN The study population included 47 patients with CIFH and 83 patients with cerebral infarction with no family history (CINFH). Control population included 100 healthy subjects with no history of ischemic stroke matched for age, sex, and ethnic background. MATERIALS AND METHODS The APOB C7673T polymorphism was analyzed by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Plasma lipids levels were also examined by standard enzymatic methods and enzyme-linked immunosorbent assay (ELISA). STATISTICS The statistical analysis was done using SPSS 14.0 software package. A P value < 0.05 was taken as significant. RESULTS This study showed an association between the APOB C7673T polymorphism and CI, especially CIFH in Han Chinese from Changsha. The T alleles frequency of the C7673T polymorphism was significantly higher in both the CIFH and CINFH groups when compared with the control group (P < 0.01; P < 0.05; respectively). And the T allele frequency in CIFH group (0.17) was higher than CINFH (0.09) group (P = 0.056). In both the CIFH and CINFH groups the serum levels of TC and LDL cholesterol were significantly high in the TT and TC genotypes than that in the CC genotype (P < 0.01), while the serum level of HDL cholesterol in the TT and TC genotypes was significantly lower than that in the CC genotype (P < 0.05). CONCLUSIONS The APOB C7673T polymorphism is related to CI, especially CIFH through changing serum lipid levels in a Chinese population.