Smoking Cessation Alters Subgingival Microbial Recolonization
Smoking Cessation Alters Subgingival Microbial Recolonization
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DOI：
10.1177/0022034509338676
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发表时间：
2009-06-01
影响因子：
7.6
通讯作者：
Kumar, P. S.
中科院分区：
文献类型：
--
作者：
Fullmer, S. C.;Preshaw, P. M.;Kumar, P. S.
戒烟改善了牙周炎的临床表现；然而，它对牙周炎的主要病原体--龈下生物膜的影响尚不清楚。这项研究的目的是通过一种定量的、不依赖培养的细菌图谱分析方法，纵向地调查戒烟是否改变了龈下微生物群落的组成。在基线、治疗后3、6和12个月收集接受牙根平整和戒烟咨询的吸烟者的牙龈下菌斑。采用末端限制性片段长度多态性(t-RFLP)分析斑块。在戒烟后6个月和12个月，吸烟者和戒烟者的微生物谱有显著差异。吸烟者的微生物群落与基线相似，而戒烟者的微生物群落特征明显不同。细菌水平的变化促成了这种转变。这些发现揭示了戒烟在改变龈下生物膜中的关键作用，并提出了一种与戒烟相关的改善牙周健康的机制。
Smoking cessation improves the clinical manifestations of periodontitis; however, its effect on the subgingival biofilm, the primary etiological agent of periodontitis, is unclear. The purpose of this study was to investigate, longitudinally, if smoking cessation altered the composition of the subgingival microbial community, by means of a quantitative, cultivation-independent assay for bacterial profiling. Subgingival plaque was collected at baseline, and 3, 6, and 12 months post-treatment from smokers who received root planing and smoking cessation counseling. The plaque was analyzed by terminal restriction fragment length polymorphism (t-RFLP). Microbial profiles differed significantly between smokers and quitters at 6 and 12 months following smoking cessation. The microbial community in smokers was similar to baseline, while quitters demonstrated significantly divergent profiles. Changes in bacterial levels contributed to this shift. These findings reveal a critical role for smoking cessation in altering the subgingival biofilm and suggest a mechanism for improved periodontal health associated with smoking cessation.