Mouse Models of Osteoarthritis: Surgical Model of Post-traumatic Osteoarthritis Induced by Destabilization of the Medial Meniscus
Mouse Models of Osteoarthritis: Surgical Model of Post-traumatic Osteoarthritis Induced by Destabilization of the Medial Meniscus
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DOI：
10.1007/978-1-0716-0989-7_14
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发表时间：
2021-01-01
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影响因子：
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通讯作者：
Otero, Miguel
中科院分区：
文献类型：
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作者：
Culley, Kirsty L.;Singh, Purva;Otero, Miguel
内侧半月板失稳(DMM)的手术模型已成为研究创伤后骨关节炎(OA)发生和发展的金标准。DMM模型模拟了临床半月板损伤，半月板损伤是人类骨性关节炎发生的已知易感因素，并允许研究疾病过程中的结构和生物变化。此外，当应用于转基因或工程小鼠模型时，这种外科手术程序允许解剖给定基因对OA启动和/或进展的相对贡献。本章描述了手术诱导小鼠模型骨性关节炎的要求，并为随后的组织学、免疫组织化学和分子分析提供了指导方针和工具。还提供了评估所选基因在转基因菌株中的贡献的方法。
The surgical model of destabilization of the medial meniscus (DMM) has become a gold standard for studying the onset and progression of post-traumatic osteoarthritis (OA). TheDMMmodel mimics clinical meniscal injury, a known predisposing factor for the development of human OA, and permits the study of structural and biological changes over the course of the disease. In addition, when applied to genetically modified or engineered mouse models, this surgical procedure permits dissection of the relative contribution of a given gene to OA initiation and/or progression. This chapter describes the requirements for the surgical induction of OA in mouse models, and provides guidelines and tools for the subsequent histological, immunohistochemical, and molecular analyses. Methods for the assessment of the contributions of selected genes in genetically modified strains are also provided.