Surface modified superparamagnetic nanoparticles for drug delivery: Interaction studies with human fibroblasts in culture
Surface modified superparamagnetic nanoparticles for drug delivery: Interaction studies with human fibroblasts in culture
复制标题
DOI：
10.1023/b:jmsm.0000021126.32934.20
复制
发表时间：
2004-04-01
影响因子：
3.7
通讯作者：
Curtis, ASG
中科院分区：
文献类型：
--
作者：
Gupta, AK;Curtis, ASG
使用磁性纳米颗粒的药物递送的概念极大地受益于以下事实：纳米技术已经发展到这样的阶段，其使得不仅可以产生具有超顺磁性的非常窄的尺寸分布范围的磁性纳米颗粒，而且可以设计颗粒表面以提供药物的位点特异性递送。纳米颗粒的尺寸和表面特性是决定颗粒在体内使用时成功的关键因素。本研究的目的是用PEG修饰磁性纳米颗粒的表面，通过抵抗蛋白质吸附和增加其细胞内摄取来改善纳米颗粒的生物相容性。在这项研究中，聚（乙二醇）（PEG）改性的超顺磁性氧化铁纳米粒子已被制备和它们对人皮肤成纤维细胞的影响进行评估，在细胞粘附/活力，形态学，颗粒摄取和细胞骨架组织的研究。已经使用各种技术来确定纳米颗粒-细胞相互作用，包括光、荧光、扫描电子显微镜（SEM）和透射电子显微镜（TEM）。纳米颗粒表面的修饰诱导细胞行为的改变，与未修饰的颗粒不同，表明细胞反应可以通过专门设计的颗粒表面来指导。(C)2004 Kluwer Academic Publishers.
The concept of drug delivery using magnetic nanoparticles greatly benefit from the fact that nanotechnology has developed to a stage that it makes possible not only to produce magnetic nanoparticles in a very narrow size distribution range with superparamagnetic properties but also to engineer particle surfaces to provide site specific delivery of drugs. The size and surface characteristics of the nanoparticles are crucial factors that determine the success of the particles when used in vivo. The aim of this study was to modify the surfaces of the magnetic nanoparticles with PEG to improve the biocompatibility of the nanoparticles by resisting protein adsorption and increasing their intracellular uptake. In this study, the poly(ethyleneglycol) (PEG) modified superparamagnetic iron oxide nanoparticles have been prepared and their influence on human dermal fibroblasts is assessed in terms of cell adhesion/viability, morphology, particle uptake and cytoskeletal organisation studies. Various techniques have been used to determine nanoparticle-cell interactions including light, fluorescence, scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The modification of nanoparticle surface induced alterations in cell behaviour distinct from the unmodified particles, suggesting that cell response can be directed via specifically engineered particle surfaces. (C) 2004 Kluwer Academic Publishers.