MiR-134 functions as a regulator of cell proliferation, apoptosis, and migration involving lung septation
MiR-134 functions as a regulator of cell proliferation, apoptosis, and migration involving lung septation
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MiR-134 作为涉及肺间隔的细胞增殖、凋亡和迁移的调节剂
DOI：
10.1007/s11626-012-9482-3
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发表时间：
2012-02-01
影响因子：
2.1
通讯作者：
Feng, Zhichun
中科院分区：
文献类型：
--
作者：
Zhang, Xiaoying;Wang, Hui;Feng, Zhichun
MicroRNAs(MiRNAs)是一种21-25个核苷酸的非编码RNA，它通过调节靶基因的表达来调节各种生物学过程，包括发育计时。决定正常出生后肺结构的一个关键因素是肺间隔，有许多miRNAs参与了发育过程。MiR-134被报道为一种强大的多能干细胞分化诱导剂，我们发现miR-134在小鼠肺间隔(从出生后2天到21天)强烈下调。进一步的细胞功能实验表明，在体外，miR-134在A549和CALU-3细胞中过表达可以促进细胞增殖，抑制细胞的凋亡和迁移能力，而在细胞中miR-134的表达下调可以起到相反的作用，提示miR-134与肺间隔有关。本研究为进一步研究其在肺发育中的作用奠定了基础。
MicroRNAs (miRNAs) are 21-25 nt long non-coding RNA that modulate various biological processes, including developmental timing via regulating the expression of their target genes. One critical determinant of normal postnatal lung architecture is septation, and there are many miRNAs involved in the development process. miR-134 is reported as a powerful inducer of pluripotent stem cell differentiation, and we have found that miR-134 is strongly downregulated during mice lung septation (from postnatal Day 2 to postnatal Day 21). Further cell function experiments have revealed that over-expression of miR-134 in A549 and Calu-3 cells can promote cell proliferation and inhibit cell apoptosis and migration abilities in vitro, and the down-expression of miR-134 in cells can act in the opposite way, which indicate that miR-134 is associated with lung septation. This study provides a basis for further investigation of its function in lung development.