A capsidless ssRNA virus hosted by an unrelated dsRNA virus
A capsidless ssRNA virus hosted by an unrelated dsRNA virus
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DOI：
10.1038/nmicrobiol.2015.1
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发表时间：
2016-01-01
影响因子：
28.3
通讯作者：
Suzuki, Nobuhiro
中科院分区：
文献类型：
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作者：
Zhang, Rui;Hisano, Sakae;Suzuki, Nobuhiro
病毒通常编码包裹其基因组的衣壳，而卫星病毒不编码复制酶，依赖辅助病毒进行复制(1)。在这里，我们报道了两种RNA病毒，yado-Nushi病毒1(YnV1)和yado-Kari病毒1(YkV1)在一种植物病原真菌Rosellinia necatrix(2)中的相互作用。YkV1与动物杯状病毒等正向单链(+)单链RNA病毒(3)具有密切的系统发育亲缘关系，而YnV1具有与真菌托蒂病毒(4)相似的完整双链(DS)RNA基因组。病毒粒子转染和感染性全长cDNA转化表明，YkV1的活性依赖于YnV1，尽管它可能编码功能性的RNA依赖的RNA聚合酶(RdRp)。免疫学和分子生物学分析表明，YkV1RNA和RdRp不仅被另一种病毒(YnV1)的衣壳蛋白反式包裹，而且YkV1促进了YnV1的积累。本研究证明了无衣壳(+)单链RNA病毒(YkV1)劫持dsRNA病毒(YnV1)的衣壳蛋白并像dsRNA病毒一样复制的相互作用。
Viruses typically encode the capsid that encases their genome, while satellite viruses do not encode a replicase and depend on a helper virus for their replication(1). Here, we report interplay between two RNA viruses, yado-nushi virus 1 (YnV1) and yado-kari virus 1 (YkV1), in a phytopathogenic fungus, Rosellinia necatrix(2). YkV1 has a close phylogenetic affinity to positive-sense, single-stranded (+)ssRNA viruses such as animal caliciviruses(3), while YnV1 has an undivided double-stranded (ds) RNA genome with a resemblance to fungal toti-viruses(4). Virion transfection and infectious full-length cDNA transformation has shown that YkV1 depends on YnV1 for viability, although it probably encodes functional RNA-dependent RNA polymerase (RdRp). Immunological and molecular analyses have revealed trans-encapsidation of not only YkV1 RNA but also RdRp by the capsid protein of the other virus (YnV1), and enhancement of YnV1 accumulation by YkV1. This study demonstrates interplay in which the capsidless (+) ssRNA virus (YkV1), hijacks the capsid protein of the dsRNA virus (YnV1), and replicates as if it were a dsRNA virus.