Oxygen-limited thermal tolerance in Antarctic fish investigated by MRI and (31)P-MRS.

Oxygen-limited thermal tolerance in Antarctic fish investigated by MRI and (31)P-MRS.
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通过 MRI 和 (31)P-MRS 研究南极鱼类的限氧热耐受性。

DOI:
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发表时间:
2002
期刊:
American Journal of Physiology. Regulatory Integrative and Comparative Physiology
影响因子:
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通讯作者:
H. Pörtner
H. Pörtner
中科院分区:
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文献类型:
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作者:
F. Mark;C. Bock;H. Pörtner

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在南极硬骨鱼Pachycara brachycephalum的氧限制的热耐受性的假设进行了测试。通过使用流通呼吸法、体内(31)P-NMR光谱和MRI,我们研究了正常氧(PO(2):20.3至21.3 kPa)和高氧(PO(2):45 kPa)下0至13 ℃之间的能量代谢、细胞内pH(pH(i))、血流和氧合。高氧降低了正常氧下观察到的代谢增量和动脉血流量的上升。正常含氧量的血流量增加在超过7摄氏度时趋于平稳,表明心血管容量有限。两组的通气努力均呈指数上升。在肝脏中,血氧增加,而在白色肌肉中,血氧保持不变(正常氧)或下降(高氧)。在两组中,pH(i)变化的斜率在低于6 ℃时遵循α-stat模式,而在高于6 ℃时则下降。总之,有氧范围在常氧下下降约6摄氏度,标志着pejus温度。通过降低循环成本,高氧改善有氧范围,但无法改变pH调节或致死极限的断点。高氧在亚致死温度下似乎是有益的,但当细胞或分子功能受到干扰时就不再有益了。
The hypothesis of an oxygen-limited thermal tolerance was tested in the Antarctic teleost Pachycara brachycephalum. With the use of flow-through respirometry, in vivo (31)P-NMR spectroscopy, and MRI, we studied energy metabolism, intracellular pH (pH(i)), blood flow, and oxygenation between 0 and 13 degrees C under normoxia (PO(2): 20.3 to 21.3 kPa) and hyperoxia (PO(2): 45 kPa). Hyperoxia reduced the metabolic increment and the rise in arterial blood flow observed under normoxia. The normoxic increase of blood flow leveled off beyond 7 degrees C, indicating a cardiovascular capacity limitation. Ventilatory effort displayed an exponential rise in both groups. In the liver, blood oxygenation increased, whereas in white muscle it remained unaltered (normoxia) or declined (hyperoxia). In both groups, the slope of pH(i) changes followed the alpha-stat pattern below 6 degrees C, whereas it decreased above. In conclusion, aerobic scope declines around 6 degrees C under normoxia, marking the pejus temperature. By reducing circulatory costs, hyperoxia improves aerobic scope but is unable to shift the breakpoint in pH regulation or lethal limits. Hyperoxia appears beneficial at sublethal temperatures, but no longer beyond when cellular or molecular functions become disturbed.
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