Macrophage activation induced by the polysaccharides isolated from the roots of Sanguisorba officinalis
Macrophage activation induced by the polysaccharides isolated from the roots of Sanguisorba officinalis
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地榆根多糖诱导巨噬细胞活化
DOI：
10.3109/13880209.2014.991834
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发表时间：
2015-01-01
影响因子：
3.8
通讯作者：
Jiang, Guiquan
中科院分区：
文献类型：
--
作者：
Tong, Haibin;Mao, Dirui;Jiang, Guiquan
背景：巨噬细胞参与免疫应答的各个阶段，是宿主防御系统的重要组成部分。多糖在药用植物中几乎无处不在，它们大多具有免疫调节和巨噬细胞活化作用。目的：研究蔷薇科地血根多糖（SOPs）对巨噬细胞活化的影响及其分子机制。材料与方法：采用水提乙醇沉淀法从山茱萸根中提取多糖。采用苯酚硫酸法、间羟基二苯法、Bradford法、气相色谱法对其理化性质进行分析。用无菌中性红法测定25、100 μ g/ml标准sop孵育RAW 264.7巨噬细胞的吞噬能力。用25、100 μ g/ml的sop孵育巨噬细胞，分别用ELISA试剂盒和Griess试剂检测tnf - α和NO的分泌。采用半定量RT-PCR检测tnf - α和iNOS转录本，Western blot检测nf - κ B信号激活情况。结果：SOPs能增强巨噬细胞对无菌中性红溶液的吞噬能力，增加tnf - α和NO的分泌。在mRNA水平上，巨噬细胞暴露于sop后，tnf - α和iNOS转录量显著增加。同时，SOPs刺激巨噬细胞诱导p65丝氨酸536位点磷酸化，I κ B表达显著降低。讨论与结论：这些结果表明，SOPs通过NF-kappa B信号通路具有显著的巨噬细胞激活特性，可以考虑作为一种新的免疫增强剂。
Context: Macrophage, involved at all stages of immune response, is an important component of the host defense system. Polysaccharides exist almost ubiquitously in medical plants and most of them possess immunomodulation and macrophage activation properties.Objective: This study elucidates the effects on macrophage activation and molecular mechanism induced by the polysaccharides (SOPs) from the roots of Sanguisorba officinalis Linne (Rosaceae).Materials and methods: Polysaccharides (SOPs) from the roots of S. officinalis were obtained by water extraction and ethanol precipitation. Physicochemical characterization of SOPs was analyzed by phenol-sulfuric acid, m-hydroxydiphenyl, Bradford method, and gas chromatography. Phagocytic capacity of RAW 264.7 macrophages incubated with SOPs (25 and 100 mu g/ml) was determined by the aseptic neutral red method. Macrophages were incubated with SOPs (25 and 100 mu g/ml), and the TNF-alpha and NO the secretion were measured using ELISA kit and Griess reagent, respectively. In addition, TNF-alpha and iNOS transcripts were evaluated by semi-quantitative RT-PCR, and NF-kappa B signaling activation was detected by Western blot assay.Results: SOPs enhanced the phagocytosis capacity of macrophages to aseptic neutral red solution and increased TNF-alpha and NO secretion. The amounts of TNF-alpha and iNOS transcript were increased significantly at the mRNA level when macrophages were exposed to SOPs. Meanwhile, the stimulation of macrophages by SOPs induced phosphorylation of p65 at serine 536 and a marked decrease of I kappa B expression.Discussion and conclusion: These results suggested that SOPs exhibited significant macrophage activation properties through NF-kappa B signaling pathway and could be considered as a new immunopotentiator.