Mechanical Regulation of Cellular Adhesion onto Honeycomb-Patterned Porous Scaffolds by Altering the Elasticity of Material Surfaces
Mechanical Regulation of Cellular Adhesion onto Honeycomb-Patterned Porous Scaffolds by Altering the Elasticity of Material Surfaces
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DOI：
10.1021/bm400202d
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发表时间：
2013-04-01
影响因子：
6.2
通讯作者：
Shimomura, Masatsugu
中科院分区：
文献类型：
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作者：
Kawano, Takahito;Nakamichi, Yuki;Shimomura, Masatsugu
在这份报告中，我们显示了蜂窝状支架细胞培养的“呼吸图”方法的制备，我们发现，它们的机械和地形性能强烈影响成纤维细胞的粘附。通过光交联的聚（1，2-丁二烯），可以成功地控制蜂窝支架的硬度没有任何表面化学变化，和支架的详细模量值通过原子力显微镜测量。我们发现，只有少量的细胞粘附在较软的蜂窝状支架上，与平膜和硬蜂窝状支架相比，其具有比传统凝胶更高的模量值。这些结果表明，弹性体蜂窝状基底可用于评估机械信号衍生的几何形状对细胞转导系统的影响。
In this report, we show the preparation of honeycomb scaffolds for cell culturing by using "breath figure" method, and we found that their mechanical and topographical properties strongly affect the adhesion of fibroblasts. By photocross-linking of the poly(1,2-butadiene), the hardness of the honeycomb scaffold can be successfully controlled without any surface chemical changes, and detail modulus values of scaffolds were measured by atomic force microscopy. We found that only small numbers of the cells adhered on the softer honeycomb scaffolds, which has even higher modulus value than conventional gels, comparing with flat films and a hard honeycomb scaffold. These results indicate that the elastomeric honeycomb substrates are useful for evaluating the effect of the mechanical signal-derived geometry on the transduction system of cells.